调宁蛋白在胆固醇结石形成中的作用及疏肝利胆方药的干预机制

The role of Calponin in cholesterol gallstone formation and effects of recipe for dispersing stagnated liver qi to promote Bile flow

目的:探讨疏肝利胆方药防治胆石病的作用机制。方法:将受试豚鼠随机分为四组,即正常组、模型组、疏肝利胆中药组、熊去氧胆酸西药组。后三组采用高胆固醇致石食饵诱发法建立豚鼠胆结石动物模型,其中疏肝利胆中药组、熊去氧胆酸西药组分别给予相应药物进行防治实验,连续7周后,分别对实验豚鼠胆结石生成情况、胆囊组织调宁蛋白(Cap)的蛋白表达进行观察。结果:疏肝利胆方药防治组能有效防止胆结石的生成(与模型组比较P<0.01),并且其效果优于熊去氧胆酸西药组(P<0.01)。模型组动物胆囊组织Cap蛋白表达水平较正常组显著升高(P<0.01),疏肝利胆中药组和熊去氧胆酸西药组胆囊组织Cap蛋白表达水平虽均高于正常组(分别为P<0.01,P<0.05),但与模型组比较,其胆囊组织Cap蛋白表达水平都呈明显降低(均P<0.01),且疏肝利胆中药组和熊去氧胆酸西药组两组胆囊组织Cap蛋白表达水平比较无显著性差异(P>0.05)。结论:疏肝利胆中药能显著降低胆结石的形成,其作用机制可能与下调胆囊平滑肌组织调宁蛋白的水平,提高胆囊平滑肌收缩能力有关。

Objective： To explore the mechanisms of the prophylacticotherapeutic function of recipe for dispersing stagnated liver qi to promote Bile flow （DSPB） on gallstone. Methods： The guinea pigs were randomly divided into 4 groups, which were normal control group, untreate group, DSPB recipe - treated group and ursodeoxycholic acid （UDCA） - treated group.The guinea pigs of the latter 3 groups were induced gallstones by high - cholesterol diet. UDCA and DSPB recipe were given to the guinea pigs in the corresponding group respectively for seven weeks. The gallstone formation and the expression of Calponin in gallblader were observed. Besuhs： The DSBP recipe was able to prevent the formation of gallstone significantly （ P 〈 0.01, vs untreated group）, which was more effective than UDCA be （ P 〈 0.01 ） ,The expression of Calponin in untreated group was significantly increased as compared with that in normal control （ P 〈 0.01 ） . Although the expression of Calponin in DSPB recipe - treated group or UDCA - treated group was increased as compared with that innonnal control grop （ P 〈 001, P 〈 0.05 ）, they were decreased as compared with that in untreated group （ P 〈 0.01 ） .There were no significant difference on the expression of Calponin between DSPB recipe - treat group and UDCA - treated group （ P 〉 0.05） .Conclusions： DSPB recipe may decrease the gallstone formation significantly.Its mechanism may be related to down - regulating the expression of Calponin in the smooth muscle of gallbladder and enhance the contractibility of smooth muscle in gallbladder.