大鼠脂肪条带结构针刺镇痛效应特异性的生物学基础

Biological basis of specifical analgesia effect following fatty strap structure acupunctured in rats

目的:观察大鼠脂肪条带结构针刺镇痛效应特异性的生物学基础。方法:选择大鼠胃经沿线脂肪条带结构上后三里以及脂肪条带结构旁两个对照位点(A1、A2),分别进行针刺,针刺结束后立即断头处死,分离出胃经沿线脂肪条带结构,制备匀浆液,采用放免法测定样本中的β内啡肽(βEP)、强啡肽A(DynA)、P物质(SP)含量。结果:βEP针刺后三里(19.08±7.42)与针刺A1、A2位点(2.27±1.99)经成组t检验比较,两者差异显著(t=5.5374,P<0.01)。DynA针刺后三里(23.08±8.61)与针刺A1、A2位点(2.19±1.81)经成组t检验比较,两者差异显著(t=5.8194,P<0.01);SP针刺后三里(1.57±0.49)与针刺A1、A2位点(1.05±0.60)经成组t检验比较,两者差异不显著(t=1.6330,P>0.05)。结论:针刺正常大鼠后三里腧穴,可使胃经沿线脂肪条带结构上特异性针刺镇痛效应物质βEP、DynA含量升高。

Objective： To study the biological basis of specifical analgesia effect by acupuncturing fat-stripe - like structure in rats. Methods： Tsu san li （st 36 ） was acupunctured on the fatty strap structue along the Stomach Meridians in rats, and another two points （A1, A2） beside st 36 were selected to be aeupunctured as control.The rats were killed by dissecting head after acupunctured, and the fatty strap structures were enucleated and homogenated. Radioimmunology assay was applied to determine the contents of β-endor- phin （β-EP）, Dynorphin A （DynA）, substance P （SP） in samples.Results： Unpaired t-test anylasis showed β-EP level was differently higher following st36 acupunctured than A1, A2 （ 19.08 ± 7.42 versus 2.27 ±1.99; t = 5.5374, P 〈 0.01 ）, DynA was significantly higher following st36 acupunctured than A1, A2 （23.08 ±8.61 versus 2.19 ±1.81; t =5.8194, P 〈 0.01）, but the difference betweent st36 and A1, A2 acupunctured did not reach the statistical significance （1.57 ± 0.49 versus 1.05 ±0.60; t = 1.6330, P 〉0.05） .Conclusions： Specifical analgesia effect substances β-EP and DynA increase in fatty strap structure along the Stomach Meridians following st 36 acupunctured in rats.