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EVA-g-NIPAAm用于控制酶活性的研究

Study on the Application of EVE-g-NIPAAm in Controlling ACtivity of Immobilized Enzyme
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摘要 采用混合接枝法和两步接枝法,将活性基团羧基引入EVA-g-NIPAAm的接枝层,以脲酶作为模型酶,利用酶中某些基团跟羧基的反应,将酶固定于该接枝热敏性材料上.当反应体系温度高于接枝物的LCST时,凝胶收缩,酶的活力被部分关闭,反应减慢;而当反应体系温度低于LCST,凝胶溶胀,酶的活力又增加,反应加快. Mixed grafting and two-step pre-irradiation grafting were used to create a kind of reactive group in the grafting layer of EVA-g-NIPAAm。With such a kind of reactive group,urease was immobilized as model enzyme on the grafting layer of EVA-g-NIPAAm.It has been shown that enzyme activity is “shut off” when the gel is raised above its LCST.This phenomenon is reversible and the immobilized enzyme regains activity below its LCST.Such catalytic hydrogels may be used to control reaction rates and temperatures with a thermal feedback mechanism.
出处 《上海交通大学学报》 EI CAS CSCD 北大核心 1996年第7期28-33,共6页 Journal of Shanghai Jiaotong University
关键词 辐射接枝 热敏性 水凝胶 固定化酶 EVA NIPAAM radiation grafting thermosensitive hydrogel immobilized enzyme
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