摘要
The detection of protein expression of clusterin and Ki-67 and the status of cell apoptosis in bladder transitional cell carcinoma; Kinetics of 5-ALA induced protoporphyrin IX in bladder cancer cell line BTU-87： an in vitro study;Mucl and muc7 gene expressions in bladder transitional cell carcinoma; The effects of protein kinase C alpha cDNA on the expression of genes of multidrug resistance in renal cell carcinoma 786- 0 cell line; Construction and sequence analysis of eukaryotic expression vector of renal cell carcinoma G250 antigen gene;Clinical study on the effect of intravesical instillation of antifibrinolytic agents with bacillus Calmette Guerin in preventing bladder cancer recurrence;Expression and significance of neuronal nitric oxide synthase in ureteropelvic junction obstruction;Comparison of intravesical bacillus Calmette- Guerin versus mitomycin C for the prevention of recurrence of superficial bladder cancer and their toxicity： meta-analysis;……
6.1 Kidney,ureter,bladder 206277 The detection of protein expression of clusterin and Ki-67 and the status of cell apoptosis in bladder transitional cell carcinoma/Chen Wei（陈炜,Dept Urol,1st Affil Hosp,Sun Yat-sen Univ,Guangzhou 510080）…∥Chin J Surg.-2006,44（2）.-111～114Objective To investigate the expression of clusterin protein in bladder transitional cell carcinoma （BTCC） and it’s association with tumor cell proliferation and apoptosis.Methods A tissue microarray （TMA） containing 87 informative cases of BTCCs was constructed firstly.The methods of immunohistochemistry and terminal deoxynucleotidyl transferase-mediated nick end-labeling were then used to examine the expression of clusterin and Ki-67 protein and the status of cell apoptosis in BTCC,respectively,and the correlations between different markers and the clusterin expression associated with patients’ clinico-pathological features were evaluated.Results In TMA of 87 BTCCs,37 （43%） cases were observed overexpression of clusterin.A significant association of clusterin expression with BTCC’s pathological grade,as well as with tumors clinica stage was observed （P<0.01）,where the frequency of overexpression of clusterin in poor differentiated BTCCs （G3,71%） and tumors in more advanced stage （T 2-4,62%） was significantly higher than that in well differentiated BTCCs （G 1-2,29%） and tumors in early stage （T a-1,28%）.In addition ,a significant correlation between clusterin expression and tumors apoptotic index （AI） was evaluated （P<0.01）,in which 57% of BTCCs with overexpression of clusterin were observed a lower AI,while 72% of tumors with normal expression of this protein showed a higher AI,but no correlation between clusterin and Ki-67 expression.Conclusion The overexpression of clusterin is associated positively with BTCC’s malignant clinical phenotypes including tumor’s differentiation and invasive depth,and it is correlated inversely with AI of tumor cells.13 refs,4 figs,2 tabs.
