三种脾气虚证模型大鼠血清胃泌素及胃窦G细胞的比较研究

Comparative Study on Serum Gastrin and G-cell in Gastric Antrum of Three Rat Models of Syndrome of Deficiency of Spleen-QI

目的:对3种脾气虚证模型大鼠的血清胃泌素及胃窦部胃泌素分泌细胞(G细胞)进行比较研究。方法:动物随机分为4组,即对照组、大黄组、双因素组(大黄法、游泳力竭法复合)、三因素组(大黄法、游泳力竭法、饥饿法复合)。检测血清胃泌素,胃窦G细胞等指标。结果:双因素组、三因素组的上述指标与对照组比较,差异有统计学意义(P<0.05,P<0.01),以三因素组为著(P<0.01)。结论:脾气虚证大鼠胃窦G细胞数量减少,释放胃泌素减少,血清胃泌素水平降低;三因素复合法制作脾气虚证动物模型效果似最优。

Objective： To make comparative study on serum gastrin （SG） and gastrin secretory cell （G-cell） in gastric antrum （GA） of three rat models of syndrome of deficiency of spleen-QI. Methods： Animals were randomly divided into four groups in terms of control group, rhubarb group, double factors group （combination of rhubarb method and swimming exhausting energy method） and triple factors group （combination of rhubarb method, swimming exhausting energy method and starvation method）. The indexes such as Serum gastrin and G-cell in gastric antrum （GA） et al were detected. Results： Compared with control group, the variances of above-mentioned indexes in double factors group and triple factors group have statistical significance （ P 〈 0.05, P 〈 0.01）, especially the triple factors group （ P 〈 0.01 ）. Conclusion： In rat models of syndrome of deficiency of spleen-QI, the amount of G-cell in gastric antrum is reduced, as well as the releasing of gastrin, and the lever of serum gastrin is declined. Using triple factors combination method to make animal models of syndrome of deficiency of spleen-QI, its effect is outweighing the other two methods.