高效液相色谱法测定小儿平喘合剂中盐酸麻黄碱的含量

Determination of ephedrine in Xiaoerpingchuan mixture by HPLC

目的建立小儿平喘合剂中盐酸麻黄碱的含量测定方法。方法采用HPLC，以ZORBAX SB-C18为色谱柱，乙腈-0．2％磷酸溶液（3：97）为流动相，检测波长210nm。结果试验表明，盐酸麻黄碱在0．06—0．96μg范围内呈良好的线性关系（r=1．000），平均回收率为99．41％，（RSD=0．65％）。结论本方法简便，准确，可作为该制剂的制控标准。

OBJECTIVE To establish the method for determination of ephedrine in Xiaoerpingchuan mixture. METHODS HPLC methd was selected to determine the content. The analysis column was ZORBAX SB-C18. The mobile phase was acetonitrile- 0.2% phosphric acid solution（ 3：97 ）. The detection wavelength was 210nm. RESULTS The calibration curve was liner in the range of ephedrine from 0.06 -0.96μ g（ r = 1. 000） ,The Average recoveries was 99.41% （ RSD =0.65% ）. CONCLUSION The method was simple and accureate. The method is suitable for the quality control of this preparation.