利用重组大肠杆菌生产聚-3-羟基丁酸的研究

Study on production of poly(3-hydroxy-butyric acid) by recombinant escherichia,coli strain

利用携带能合成聚-3-羟基丁酸的基因的大肠杆菌E．coli XL1-Blue,优化培养基和培养条件后,进行了补料分批培养。结果表明,重组大肠杆菌E．coli XL1-Blue(pKSS105)的最适培养基为R培养基。在最佳条件下,以葡萄糖为唯一碳源培养工程菌60h后,发酵液中菌体干重达183g/L,P(3HB)的产量为133．8g/L,P(3HB)含量为73．1%。实验结果为P(3HB)实际应用提供了可能性的基础。

The aim of this study is to build up the optimum cultivation conditions of E. coli XL1-Blue which haboured the P（3HB） synthetase gene. And feed-bach culture of the strain was carried out in the fermentoe The results show that the optimum medium on which recombinant E. coli XL1-Blue（pKSS105） was cultivated is mineral salts R medium plus glucose as sole carbon source, and the final cell dry weight, P（3HB） yield and P（3HB） content were 183g/L, 133.8g/L, and 73.1%, respectively in a 20L fermentor after 60 hours of feed-bach culture in the optimum condition.