电针治疗对睾丸功能损害雄性大鼠精子数量和质量的影响

Influence of Electroacupuncture on Sperm Count and Quality in Oligoasthenozoospermic Rats

【目的】探讨电针治疗对睾丸功能损害雄性大鼠精子数量和质量的影响。【方法】SD雄性大鼠34只,除正常组10只外,其他大鼠采用腺嘌呤灌胃复制睾丸功能损害模型,存活大鼠随机分为电针组(N=8)和模型组(N=8);电针组针刺关元、肾俞及三阴交穴。采用计算机辅助精子质量分析(CASA)系统对大鼠精液进行分析。精子质量测定指标包括精子密度、精子活率及前向运动精子百分率。【结果】与正常组比较,模型大鼠精子密度、精子活率及前向运动精子百分率均显著降低(P<0.01);电针组与模型组比较,各指标均显著升高(P<0.05)。【结论】电针关元、肾俞及三阴交穴可以改善腺嘌呤所致睾丸功能损害大鼠的精液质量。

[ Objective ] To observe the effect of electroacupuncture （EA） on sperm count and quality in oligoasthenozoospermic rats. [ Methods] Twenty-four male SD rats were given adenine by intragastral administration to establish oligoasthenozoospermic models. After the modeling, the survival rats were randomized into EA group （ N = 8） and model group （N = 8）. EA group was treated with EA of Guanyuan （CV4）, Shenshu （BI23） and Sanyinjiao （SP6） acupoints. Other 10 rats served as the normal controls. Sperm samples were analyzed by computer-aided semen analysis system （CASA） and the observed sperm parameters included sperm concentration, semen viability and percentage of forward progressive sperm. [ Results] Compared with the normal group, sperm concentration, semen viability and percentage of forward progressive sperm were much decreased in the model group （ P 〈 0.01 ）, and EA increased the above parameters （ P 〈 0.05 compared with the model group）. [ Conclusion ]EA of Guanyuan （CV4）, Shenshu （BI23） and Sanyinjiao （SP6） acupoints can improve sperm quality in oligoasthenozoospermic rats.