骨髓间充质干细胞分离及条件培养基在其培养扩增中的作用

Isolation of Rat Marrow Mesenchymal Stem Cells and Effect of Conditioned Medium on Its Proliferation

【目的】建立一种简便、快速、实用的大鼠骨髓间充质干细胞(mesenchymal stem cells,MSCs)体外分离、纯化和扩增的方法。【方法】采用贴壁法分离纯化大鼠骨髓MSCs(rMSCs),并对其形态学特征进行观察,培养时观察条件培养基对rMSCs生长的影响。【结果】贴壁法能有效地分离rMSCs,采用条件培养液培养,细胞活力好,增殖能力强,对维持细胞正常形态有着重要作用。【结论】贴壁分离法能成功地进行rMSCs的体外分离,采用条件培养液建立的rMSCs培养方法有效可行。

[Objective] To establish a simple, rapid and practical method for the in-vitro isolation, purification and proliferation of rat marrow mesenchymal stem cells （MSCs）. [ Methods ] Rat MSCs were isolated and purified by differential adhesion method. The effects of conditioned medium on the morphologic characteristics and growth of rat MSCs were observed. [ Results] Rat MSCs were effectively purified by differential adhesion method, and grew well with good proliferation and normal morphology in conditioned medium. [ Conclusion ] Differential adhesion method and conditioned medium are effective for the purification and culture of rat MSCs.