摘要
抗轮状病毒IgY可用两步盐析结合凝胶过滤从蛋黄中分离出来,用SDS-PAGE检测其纯度可达到95%以上。纯的IgY经胃蛋白酶分解得到的抗体片断(Fab),经SDS-PAGE和MALDI质谱法测定,其纯度达到99%以上。结果表明,所设计的分离抗轮状病毒IgY和Fab的方法简单、有效。经ELISA法检测,Fab的活性仍保持在IgY原始活性的70%以上。
Human Rotavirus (HRV) were used as antigen to immunize laying chickens and anti-HRV IgY was isolated from the yolk of eggs The purity was over 95 % by antibody fragment Fab' was methods, and its purity was isolating hen antibodies (IgY activity of IgY determined by using two salting-out steps followed by one-gel fihration chromatography methods. SDS-PAGE analysis. The purified IgY was digested with pepsin and the smaller obtained. The purified Fab' was characterized by SDS-PAGE and MALDI MS over 99%. The results indicate that the methods designed in this paper for and Fab') are simple and effective, the retained Fab' is over 70% of the initial the ELISA method.
出处
《化学工业与工程》
CAS
2006年第4期317-319,共3页
Chemical Industry and Engineering