不同配比黄芪汤干预大鼠胆汁淤积性肝硬化作用观察

Effect of Different Ratio of Huangqi Decoction on Cholestatic Cirrhosis in Rats

目的：观察不同配比的黄芪汤（3：1、6：1、9：1）干预大鼠胆汁淤积性肝硬化的药理作用。方法：结扎大鼠胆总管制备大鼠胆汁淤积性肝硬化模型；结扎1周后，将模型大鼠随机分为模型组和药物干预组，药物干预分别给予不同配比的黄芪汤经口灌胃，模型组给予等量生理盐水；用药4周后杀鼠取材。观测内容包括大鼠一般状况、组织病理、肝功能（血清ALT、AST、ALP、GGT活性及TBil、TP、Alb的含量）、肝组织羟脯氨酸（HyP）含量。结果：造模5周时大鼠胆汁淤积性肝硬化完全形成，与假手术组大鼠比较，模型组大鼠血清GGT、ALP活性及血清TBil含量、肝组织Hyp含量显著升高（P＜0．05-0．01V8sham），血清TP、Alb含量显著下降（P＜0．05—0．01 vs sham）。与同期模型对照组比较，黄芪汤（6：1、3：1）干预治疗组肝纤维化程度显著减轻，血清AlJP、GGT活性、TBil含量及肝组织Hyp含量显著降低（P＜0．05—0．01 vs 5W model）。结论：黄芪汤（6：1、3：1）有不同程度的干预大鼠胆汁淤积性肝硬化形成的作用。

Objective： To investigate the effect of different ratio of Huangqi Decoction （ 3 ： 1,6 ： 1,9 ： 1 ） on cholestatic cirrhosis in rats. Method：Rat cholestatic cirrhosis was duplicated by ligation of bile duct. After 1 week rats were divided into model control group and different ration of Huangqi Decoction groups. The oral administration of Huangqi Decoction lasted for 4 weeks, and then rats were sacrificed for determination of liver function,liver tissue Hyp conten and histological changes. Results： cholestatic cirrhosis was successfully duplicated after 5 weeks modeling. Compared with that of normal group, serum GGT and ALP activity, serum TBiL content and liver tissue Hyp content in model group increased significantlly（ P 〈 0.05 - 0.01 vs sham） ,while serum TP and Alb content decreased significantly. Compared with that of model group, degree of cirrhosis in groups of ratio 3 ： 1 and 6 ： 1 of Huangqi Decoction alleviated significantly, serum ALP and GGT activity, serum TBiL content and liver tissue Hyp content decreased significantly （ P 〈 0.05 - 0.01 vs 5W model）. Conclusion： The ratio of 3 ： 1 and 6 ： 1 of Huangqi Decoction inhibits formation of cholestatic cirrhosis effectively.