期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

生物功能化半导体量子点对活细胞内蛋白质分子可视化的研究进展 被引量：4

Advances in studies on visualizing of proteins in a living cell with bio,functionalized semiconductor quantum dots
原文传递
导出
摘要 半导体量子点(或称半导体纳米微晶体)具有独特的光谱特性和良好的光化学稳定性。通过改变量子点的材料或粒径大小可在同一波长光激发下获得从紫外到近红外(或从蓝色到红色)波长范围内任意点的发射光谱。随着近年来生物亲和性功能化纳米技术的发展,为半导体量子点用于多通道、高通量对活细胞内蛋白质分子直接观察研究这一国际未解决的难题提供了可能。概述了生物功能化半导体量子点在活细胞生理条件对蛋白质分子进行可视化研究的最新进展,评价了其作为荧光探针对活细胞蛋白质分子进行实时动态研究的发展前景。 In comparison to conventional organic dyes, semiconductor quantum dots（QDs, also known as semiconductor nanocrystals） have unique spectral properties and better photochemical stability. By varying the size and composition of QDs, the emission wavelength can be tuned from the blue to the near infrared. With the development of the bio-functionalized nanotechnology, bioconjugated QDs offers possibility of simultaneous readout of multiple proteins in a living cell. This review focuses on recent advances in visualizing proteins in living cell using bioconjugated QDs. QD-based probes provides the potential to real-time monitor proteins dynamic changes in a single living cell.
作者 杨凯
机构地区 重庆医科大学附属第一医院口腔颌面外科
出处 《国际生物医学工程杂志》 CAS 2006年第3期142-144,共3页 International Journal of Biomedical Engineering
基金 国家自然科学基金资助项目(30470893)
关键词 半导体 量子点 纳米微晶体 蛋白质 细胞 semiconductor quantum dots nanocrystals protein cell
分类号 Q2-33 [生物学—细胞生物学]
  • 相关文献

参考文献19

  • 1Robert FS.High-speed biologists search for gold in proteins[J].Science,2001,294(5549):2074-2077.
  • 2Alivisatos P.The use of nanocryatals in biological detection[J].Nat Biotechnol,2004,22(1):47-52.
  • 3Tanke HJ,Dirks RW,Raap T.Fish and immunocytochemistry:Towards visualising single target molecules in living cells[J].Curr Opin Biotechnol,2005,16(1):49-54.
  • 4Michalet X,Pinaud FF,Bentolila LA,et al.Quantum dots for live cells,in vivo imaging,and diagnostics[J].Science,2005,307(5709):538-544.
  • 5Alivisatos P.The use of nanocrystals in biological detection[J].Nat Biotechnol,2004,22(1):47-52.
  • 6Chan WCW,Maxwell DJ,Gao XH,et al.Luminescent quantum dots for multiplexed biological detection and imaging[J].Curr Opin Biotechnol,2002,13 (1):40-46.
  • 7Chan WC,Nie S.Quantum dot bioconjugates for ultrasensitive nonisotopic detection[J].Science,1998,281(5385):2016-2018.
  • 8Bruchez M J,Moronne M,GinP,et al.Semiconductor noncrystals as fluorescent biological labels[J].Science,1998,281(5385):2013-2016.
  • 9Thomas MJ.Quantum dots finally come of age[J].Nat biotechnol,2003,21(1):32-33.
  • 10Gao X,Yang L,Petros JA,et al.In vivo molecular and cellular imaging with quantum dots[J].Curr Opin Biotechnol,2005,16(1):63-72.

二级参考文献9

  • 1戴荣禧,徐国江,刘莲英,林秀媛,沈慧屏,周筱刚,高彤,王亚辉.天花粉蛋白对滋养层细胞专一损伤机制的研究[J].实验生物学报,1993,26(4):411-427. 被引量：14
  • 2[1]Bruchez Jr. M., Moronne M., Gin P. et al.. Science[J], 1998, 281: 2 013-2 016
  • 3[2]Chan W. C. W., Nie S. M.. Science[J], 1998, 281: 2 016-2 018
  • 4[3]Murray C. B., Norris D. J., Bawendi M. G.. J. Am. Chem. Soc.[J], 1993, 115: 8 706-8 715
  • 5[4]Peng X. G., Wickham J., Alivisatos A. P.. J. Am. Chem. Soc.[J], 1998, 120: 5 343-5 344
  • 6[5]Rogach A. L., Nagesha D., Ostrander J. W. et al.. Chem. Mater.[J], 2000, 12: 2 676-2 685
  • 7[7]Klayman D. L., Griffin T. S.. J. Am. Chem. Soc.[J], 1973, 95: 197-199
  • 8[8]Rajh T., Micic O. I., Nozik A. J.. J. Phys. Chem.[J], 1993, 97: 11 999-12 003
  • 9[9]Lakowicz J. R.. Principles of Fluorescence Spectroscopy[M], New York: Plenum Press, 1983: 187-256

共引文献109

同被引文献28

  • 1杨凯.半导体量子点荧光探针在恶性肿瘤中的应用[J].国外医学（肿瘤学分册）,2005,32(11):836-838. 被引量：5
  • 2杨凯,陈睿,孙德平.半导体量子点对口腔鳞癌BcaCD885细胞生长、侵袭和转移能力影响的体外研究[J].重庆医科大学学报,2007,32(4):337-339. 被引量：2
  • 3Robert FS. High-speed biologists search for gold in proteins[J]. Science, 2001, 294(5549) : 2074-2077.
  • 4Alivisatos P. The use of nanocrystals in biological detection[J]. Nat Biotechnol, 2004, 22 (1) : 47-52.
  • 5Michalet X, Pinaud FF, Bentolila LA, et al. Quantum dots for live cells, in vivo imaging, and diagnostics[J]. Science, 2005, 307 (5709) : 538-544.
  • 6Jaiswal JK, Mattoussi H, Mauro JM, et al. Long-term multiple color imaging of live cells using quantum dot bioconjugates[J]. Nat Biotechnol, 2003, 21 (1):47-51.
  • 7Chan WC, Maxwell DJ, Gao X, et al. Luminescent quantum dots for multiplexed biological detection and imaging [J]. Curt Opin Biotechnol, 2002, 13 (1) :40-46.
  • 8Tada H, Higuchi H, Wanatabe TM, et al. In vivo real-time tracking of single quantum dots conjugated with monoclonal anti-HER2 antibody in tumors of mice[J]. Cancer Res, 2007, 67 (3) : 1138-1144.
  • 9Gao X, Yang L, Petros JA, et al. In vivo molecular and celhdar imaging with quantum dots[J]. Curr Opin Biotechnol, 2005, 16 (1) : 63-72.
  • 10Heldin CH, Miyazono K, ten Dijke P. TGF-beta signalling from cell membrane to nucleus through SMAD proteins [J]. Nature, 1997, 390 (6659) : 465-471.

引证文献4

二级引证文献8

国际生物医学工程杂志
2006年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部