国产碱性脂肪酶的测定方法及特性研究

Studies on Mensuration and Characteristics of Indigenous Alkaline Lipase

对几种国产脂肪酶的酶学特性进行了研究,并以酸碱滴定法对碱性脂肪酶的测定方法进行了探讨。结果表明:底物的乳化均匀程度对酶活力的测定有一定的影响,在α为0.05、经T检验得到:乳化、非乳化条件下及乳化液和缓冲液分开吸取和混合液乳化后吸取存在显著差异。SZ酶、SZX酶、SH酶在相同底物浓度下的最佳作用pH分别为9.0、8.9、9.1;最佳作用温度为35℃、35℃和45℃;达到最高活性所用的反应时间分别为15、10和10min。

Enzymatic characteristics of several indigenous alkaline lipases （SZ. SZX. SH） were studied in this paper and the assay method of alkaline lipase was discussed. The result showed that emulsification degree of substrate affected enzyme activity. Compared emulsification method with non-emulsification method by T-test （α=0.05）, the two methods existed distinct difference. Compared two drawing methods of emulsion and buffer liquid, which also existed difference. The optimum pH of indigenous alkaline lipases （SZ. SZX. SH） were 9.0, 8.9, 9.1; Optimum reaction temperatures were 35 ℃, 35 ℃ and 45 ~C; Optimum reaction time of the three enzymes were 15 min. 10min and 10min under the same substrate.