摘要
目的:探讨Wnt/β-catenin信号转导通路与肿瘤发生的关系,为肝癌的防治提供新的思路。方法:选取Wnt/β-catenin信号转导通路中上下游关键因子wnt1、β-catenin、APC、cyclinD1以及c-myc等,应用RT-PCR法观察它们在正常肝脏,不典型增生肝脏和肝癌组织中的转录水平。用免疫组化染色法研究β-catenin、APC和cyclinD1等3个因子有无表达。结果:在正常肝脏中,用RT-PCR未检测到wnt1、cyc-linD1以及c-myc的mRNA转录,免疫组化染色也只观察到β-catenin在细胞膜处有比较弱的表达。诱癌14周后,在发生不典型增生的肝组织中,检测到β-catenin、APC和cyclinD13个基因的转录。通过免疫组化染色也观察到β-catenin蛋白在胞质中的积累,APC和cyclinD1在部分细胞中出现表达。诱癌16周后,在肝癌组织中,除wnt1mRNA外,其他几个因子的mRNA都有转录,免疫组化也印证了上述各个发生转录的因子在蛋白水平都有不同程度的表达。结论:Wnt/β-catenin信号转导通路在大鼠的肝癌形成过程中被激活,其可能是实验性肝癌发生的原因之一。
AIM： To investigate the role of Wnt/β-catenin signaling transduction pathway in rat hepatocarcinogenesis. METHODS： The mRNAs of Wnt1, β-catenin, APC, cyclin D1 and c-myc genes were amplified by using of semiquantitative reverse transcription polymerase chain reaction （RT-PCR） from normal rat livers, atypical hyperplasia livers and hepatoma tissues, respectively. Then the proteins expression of β-catenin, APC and cyclin D1 was examined by immunohistochemical staining. RESULTS： In normal rat livers, the mRNAs of Wnt1, cyclin D1 and c-myc genes were not detected and only β-catenin protein was observed to have low expression at cellular membrane. However, 14 weeks after cancer induction in atypical hyperplasia livers, β-catenin protein and APC protein were accumulated in cytoplasm. Meanwhile, cyclin D1 protein was detected in cytoplasm and/or nucleus in some cells. 16 weeks after cancer induction in hepatoma tissues, the mRNAs and protein expression of β-catenin, APC, cyclin D1 and c-myc genes were detected except Wnt1. CONCLUSION： The activation of Wnt/β-catenin signaling transduction pathway might be one of the reasons for rat hepatocarcinogenesis.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2006年第4期454-457,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
纽约中华医学基金会(CMB
82-412)
