阿托伐他汀对移植动脉硬化抑制作用的实验研究

Depressant effect of atorvastatin on arteriosclerosis of aortic allograft in rats

目的研究阿托伐他汀(atorvastatin)对大鼠移植动脉硬化的抑制作用。方法建立大鼠腹主动脉移植模型。动物分为3组:(1)异系移植对照组;(2)异系移植实验组;(3)同系移植对照组。6 0 d后对移植动脉行病理组织学检测,观察移植动脉内膜增生程度,并行免疫组织化学染色(SP法)检测移植动脉增殖细胞核抗原(PCNA)和平滑肌肌动蛋白(α-SMA)的表达情况。结果异系移植实验组血管内膜增厚程度显著低于异系移植对照组,(1),(2),(3)组移植动脉内膜增厚面积比分别为(3 5.2 0±6.3 5)%,(1 2.4 0±2.6 5)%,(1.2±1.1 0)%,差异均有统计学意义(P<0.05)。PCNA在异系移植实验组表达明显低于异系移植对照组和高于同系移植对照组,(1),(2),(3)组分别为(1 7.2 0±1.6 5)%,(5.4 0±0.8 0)%,(1.1 0±0.2 5)%,差异有统计学意义(P<0.05)。结论阿托伐他汀可抑制大鼠移植动脉硬化,PCNA基因表达的下调可能是阿托伐他汀抑制移植动脉硬化的机制之一。

Objective To study the depressant effect of atorvastatin on arteriosclerosis of aortic allograft in rats. Methods The models of abdominal aorta transplantation were established in rats with the use of micro-surgery. The recipients were divided into three groups： allograft control group, allograft experimental group and isograft control group. After 60 days of transplant, vascular intimal thickness （ VIT ） in all of the groups was observed by histological examination. The expression of PCNA and α-SMA was determined by immunohistochemistry. Results The degree of VIT in rats of the allograft experimental group was lower than thai in the allograft control group; the VIT area ratio in the allograft control group, allograft experimental group and isograft control group was （12.40±2.65）% , （5.20 ±6.35）% , and （1.2 ±1. 10）% , respectively, A statistical difference between these groups was observed （ P 〈 0. 05 ） . The expression level of PCNA was lower in allograft experimental group （ 5. 40 ±0. 80 ） % than that in allograft control group （ 17.20 ±1.65 ） % （ P 〈 0.05 ） . Conclusions The expression of PCNA in the transplant aorta and the arteriosclerosis of aortic allograft could be suppressed by atorvastatin.