实验性脑出血微创抽吸术的建立与评估

Establishment of minimally invasive aspiration in intracerebral hemorrhage

目的在实验性脑出血模型基础上实施微创血肿抽吸术并对其进行评估。方法将Ⅳ型胶原酶注人SD大鼠尾状核诱导形成脑出血10h后,向血肿腔内注入尿激酶溶解血凝块,实施微创血肿抽吸术排出脑内血肿。通过对血肿抽吸组与未抽吸组及似手术组之间脑血肿体积、脑组织含水量的测定及行为学评分,对微创血肿抽吸术的效果进行评估。结果脑出血后抽吸组脑组织血肿体积小于对照组(P＜0．01),腑组织含水量抽吸组和对照组差异无显著性(P＞0．05),脑出血后第3天和第7天抽吸组的神经功能恢复优于对照组(P＜0．05)。结论在胶原酶诱导的大鼠脑出血模型的基础上经尿激酶溶解血块后实施微创血肿清除术能较好地清除脑内血肿,促进急性期神经功能恢复。

Objective To establish and evaluate minimal invasive hematoma aspiration on the experimental intracerebral hemorrhage models. Methods Intracerebral bemorrhage was induced by injecting Ⅳ collagenase into caudate nucleus of SD rats, and after ten hours urokinase was injected into hemotoma intracavity to dissolve clot. Hemotoma was removed from intracavity by use of minimal invasive hematoma aspiratian. The effect of minimal invasive hematoma aspiration was evaluated by determination of hematoma volume and brain water, as well as ethology score, among hemotoma aspiration group, bemotoma group and sham operation group. Results Brain bemotoma of aspiration group was smaller than that of control group after cerebral hemorrhage （ P 〈 0.01 ）. There was no significant difference in brain water between aspiration group and control group （ P 〉 0.05 ）. Neuro - functional recovery of aspiration group was better than that of control＇ s on third day and seventh day after intracerebral hemorrhage （ P 〈 0.05 ）. Conclusion In the rat model of intracerebral hemorrhage , the use of minimal invasive hematoma aspiration after dissolving clot could remove hematoma better and promote nettro-functional recovery during acute stage.