氯胺酮对大鼠隔内侧核和斜角带核垂直支一氧化氮合酶表达的影响

Effects of ketamine on expression of nitric oxide synthase in medial septal nuclei and vertical diagonal band of rats

目的:探讨不同剂量的氯胺酮对大鼠隔内侧核(MS)和斜角带核垂直支(VDB)一氧化氮合酶(NOS)表达的影响。方法:将40只大鼠随机分为正常组、生理盐水(10ml·kg-1,ip)组、氯胺酮低、中、高(25、50、100mg·kg-1,ip)剂量组,各组分别按上述设定剂量,于晚上21时给药,每天1次,连续7d,于末次注射后2h,灌注、固定、切片,采用还原型尼克酰胺腺嘌呤二核苷酸脱氢酶(NADPH-d)法显示MS、VDB背侧部和腹侧部内的NOS表达。结果:氯胺酮各剂量组大鼠MS、VDB背侧部和腹侧部内的NOS阳性神经元数量比正常组、生理盐水组减少(P<0.05或P<0.01),各剂量组之间比较差异也有统计学意义(P<0.05);NOS阳性神经元染色变淡,细胞平均灰度值增加,氯胺酮高剂量组与低剂量组相比差异有统计学意义(P<0.01)。结论:氯胺酮可剂量依赖性地抑制MS和VDB内NOS的表达,提示这可能与氯胺酮的麻醉效应和精神毒副作用有关。

AIM： To explore the effects in different dosage of ketamine on the expression of nitric oxide synthase （NOS） in medial septal nuclei （MS） and vertical diagonal band （VDB） of rats. METHODS： 40 SD rats were randomized into normal control group, normal saline（NS） group, low, middle and high dosage of ketamine（25,50,100 mg·kg^-1 ,ip） group. Each group was maintained intraperitoneally by given daily dosage once a day for a period up to 7 days. That was done at 21 ： 00 hour each night, and by 2 h of the last administration,poured, fixed and sliced were carried out. Method of nicotinamide adenosine dinucleotide phosphate diaphorase（NADPH-d） was used to show the expression of NOS in MS and VDB pars dorsalis and ventro. RESULTS： Compared with normal control and NS group, the number of NOS positive neurons were decreased in MS and VDB pars dorsalis and ventro in ketamine groups. There were statistical meaing in different-dosage groups of ketamine （ P 〈0.05 or P 〈 0.01）. Cell-dyeing became lighten,and celluar average optical density value was increased in MS and VDB pars dorsalis and ventro in each administered ketamine groups, There were statistical meaing in high and low dosage of ketamine（ P 〈 0.01）. CONCLUSION：Ketamine can reduce the expression of NOS in MS and VDB by dosage dependence, which suggests that anesthesia effect of ketamine has possible correlation with neuropathic side effect.