摘要
目的评估谷氨酰胺(GLN)联合丝裂霉素C(MMC)对人胃癌细胞株MGC-803的作用。方法采用MTT法检测药物对细胞的作用,应用中效原理判断两药合用效果,流式细胞仪检测细胞周期分布。结果GLN无明显刺激肿瘤生长作用,但随药物浓度升高和作用时间延长,可抑制肿瘤细胞生长,并呈剂量及时间依赖性。MMC具有良好的抗肿瘤作用,呈明显的时间依赖性。GLN与MMC联用时呈较弱的拮抗作用(1<CI<1·2703)。MMC有明显的S期阻滞作用,小剂量GLN对此阻滞作用影响较小,但大剂量GLN可显著减弱S期阻滞,降低细胞增殖指数。结论GLN与MMC对胃癌细胞的疗效呈剂量相关的拮抗作用。较大剂量MMC与小剂量GLN联用效果较好。
Objective To evaluate the effect of combination of glutamine （GLN） and mitomycin C （MMC） on the human gastric carcinoma cell line MGC-803 in vitro. Methods The effects of GLN and MMC were measured by MTT assay, and the interaction between the two agents was evaluated by the median-effect principle. Flow cytometry was used for cell cycle analysis. Results GLN did not significantly stimulate the cell growth in vitro. High-concentration of GLN could inhibit the cell growth. MMC could effectively inhibit the cell growth in a time-dependent manner. The interaction of these two agents showed a weak antagonistic activity （ 1 〈 CI 〈 1. 2703 ）. MMC induced remarkable S-phase arrest. Low-dose GLN has limited effect on the S-phase arrest of MMC, while high-dose GLN significantly attenuated the S-phase arrest and lowered the proliferation index of MGC-803 cell. Conclusions Combination of GLN and MMC has a a weak and dose-dependent antagonistic activity in the treatment of gastric carcinoma cell line MGC-803. The combination of high-dose MMC and low-dose GLN may achieve better efficacy.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2006年第3期345-349,共5页
Acta Academiae Medicinae Sinicae
