人重组γ－干扰素（hrIFN－γ）对人胎盘滋养层hCG分泌和蜕膜蛋白质合成的影响

The Effect of Human Recombinant Interferon Gamma(hrIFN -γ)on hCG Secretion of Trophoblast and Protein Synthesis of Decidual Tissue In Vitro

研究证明人重组γ－干扰素（ｈｒＩＦＮ－γ）对人胎盘滋养层组织绒毛膜促性腺激素（ｈＣＧ）和蜕膜组织蛋白质合成在体外实验中均有抑制作用。结果指出ｈｒＩＦＮ－γ在剂量为２５０Ｕ／ｍｌ培液和２５００Ｕ／ｍｌ培液时明显地抑制滋养层组织ｈＣＧ分泌，与对照组比较Ｐ值分别为０．０５和０．０１，并呈剂量相关反应，同时ｈｒＩＦＮ－γ在剂量范围为１０Ｕ～１０００Ｕ／ｍｌ培液时，明显抑制蜕膜蛋白质合成３Ｈ亮氨酸掺入的ｃｐｍ值，在对照组和实验组之间无论是细胞内或是分泌蛋白都有明显差别（Ｐ＜０．０５），而且抑制作用呈剂量相关反应，ＩＦＮ－γ抗血清能阻断其抑制作用，但ＩＦＮ－α对蜕膜组织蛋白质合成无作用。

In this study the effect of human recombinant interferon gamma(hrIFN-γ)on hCG secretion of human first trimester trophoblast and protein synthesis of decidual tissue was investigated in vitro.The results indicated that hrIFN-γ at the doses of 250 U/ml medium and 2500 U/ml medium decreased hCG secretion of trophoblast obviously(p<0.05,P<0.01)in a dose dependent manner.The effect of hrIFN-γon protein synthesis at the dose of 10 U to 1,000 U/ml medium inhibited the 3H leucine incorporation obviously.The cpm values between control and experimental groups were significantly different (P<0.05)in a dose-dependent manner.Furthermore its inhibitory effect is in a dose-dependent manner and was neutralized by IFN-γ antiserum.The IFN -α at the doses used did not find any effect on protein synthesis decidual tissue.