反复自然流产绒毛生化学改变的研究

Study on the Changes of Biochemistry and Clinical Analysis of Chorionic Villi of Spontaneous Abortion

木研究对３４例反复自然流产（ＲＳＡ）、１０例１次流产及３８例正常妊娠人流绒毛组织，应用生物化学及免疫组化方法，检测绒毛组织中谷胱甘肽硫转移酶（ＧＳＴ）活性、腺苷脱氨酶（ＡＤＡ）活性和还原型谷胱甘肽（ＧＳＨ）含量及γ谷氨酸转肽酶（γ－ＧＴ）免疫组化定位进行对比性研究。结果表明，ＲＳＡ绒毛组织较正常绒毛组织中ＧＳＴ活性和ＡＤＡ活性均有明显改变（Ｐ＜０．０１）。γ－ＧＴ免疫组化定位、阳性颗粒的分布及强度也有明显的差别。自然流产３次以上较２次和１次流产的ＧＳＨ含量和ＧＳＴ活性明显降低，经统计学分析均有非常显著差别（Ｐ＜０．０１）。该结果提示绒毛组织本身某些生化功能的改变，可能是原因不明的ＲＳＡ的原因之一。

The incidence of clinically recognized pregnancies ending in spontaneous abortion is generally between 12%～15%.Spontaneous abortion is associated with conditions such as deformed uterus chromosomal abnormality,endocrine factors and immunological causes,but the cause in most cases is unknown. In order to study on mechanism of spontaneous abortion,changes of biochemistry and clinical analysis of chorionic villi of thirty-four women who suffered from recurrent abortion of unknown etiology,ten women have one time abortion and thirty-eight normal women as a control were determined. The results showed that:1.The level of glutathione-S-transferase(GST) of chorionic villi of spontaneous abortion was more markedly decreased than that of the control(P<0.01),but the level of adenosine deaminase(ADA)was more markedly increased than that of the control(P<0.01).2.Glutathione content of chorionic villi in patients who suffered three times from spontaneous abortion were higher than that in those having twice the abortion (P<0. 01).3.Immunohistochemical localization of Y-glutamyl transpeptidase(Y-GT) in chorionic villi of patients was stained more weakly than that of control.The present data suggest that the changes of biochemical properties of chorionic villi may be one of the causes of spontaneous abortion of unknown etiology.