Syk基因甲基化和肝癌术后复发转移关系的研究

The Study of Roles about Syk Promoter Methylation in Postoperative Recurrence and Metastasis of Liver Cancer

目的探讨肝癌组织Syk基因启动子甲基化改变的特点,及其与肝癌术后复发转移的关系。方法采用甲基化特异性PCR方法检测Syk基因启动子甲基化情况,结合随访结果,探讨Syk基因启动子甲基化和肝癌术后复发转移的关系。结果在64例肝癌组织标本中,有19例检测到Syk基因启动子甲基化,发生率为29.7%;在对应癌周正常肝组织中,只有2例检测到Syk基因启动子甲基化,发生率为3.1%。癌组织Syk基因启动子甲基化发生率显著增高(P<0.005)。在19例癌组织检测到Syk基因启动子甲基化的肝癌患者中,有14例术后发生癌复发转移,发生率为73.7%;在45例癌组织未检测到Syk基因启动子甲基化的肝癌患者中,只有19例术后发生癌复发转移,发生率为42.2%。癌组织检测到Syk基因启动子甲基化的肝癌患者术后复发转移发生率显著增高(P<0.05)。结论Syk基因启动子甲基化可能是肝癌发生发展过程中重要的机制之一。肝癌组织Syk基因启动子甲基化可能是预测患者术后复发转移潜在的生物标记物。

Objective To detect methylation status of the promoter region of Syk gene in liver cancer, the relationship between the aberrant methylation of Syk gene and postoperative recurrence and metastasis after resection was analyzed. Methods Using methylation-specific polymerase chain reaction technique（MSP）, specimens from 64 patients of liver cancer were detected methylation of Syk promoter region. According to follow-up, we analyzed the relationship between the aberrant methylation of Syk gene and postoperative recurrence and metastasis. Results ① Among 64 liver cancer tissue.s, methylation of Syk promoter region were found in 19 patients（29.7% ）. In contrast, methylation of Syk promoter region were found in only 2 of adjacent normal tissues （3.1% ）, the rate of methylation of Syk promoter in liver cancer tissues was higher than that of adjacent normal tissues. ②In 19 cases with Syk promoter methylation, 14 were found to have postoperative recurrence and metastasis（73.7% ）. In 45 cases without Syk promoter methylation,only 19 were found to have postoperative recurrence and metastasis （42.2 % ）. A significant difference was noted between two groups. Conclusion ① There is significant difference of Syk promoter methylation between liver cancer tissues and adjacent normal tissues.② Promoter methylation of Syk gene may serve as a potential biomarker for postoperative recurrence and metastasis of liver cancer patients.