克氏原螯虾热休克蛋白70ku类似物基因的cDNA分析

Sequence analysis of 70 ku heat shock protein cognate cDNA in crayfish

根据已测序的克隆号PCI246基因序列设计2条引物,用快速扩增cDNA末端技术扩增克氏原螯虾(Procambarus clarkii)70 ku热休克蛋白同类物基因cDNA的5'端片段和3'端片段,测序得到1 184 bp基因片段,包含该基因的完整3'端,GenBank登录号AY357302,与GenBank序列号AB016836家蚕(Bombyx mori)的70 ku热休克蛋白同类物mRNA的部分片段83%同源。和果蝇(Drosophila melanogaster)的热休克蛋白同类物3的同源性最高(GenBank的登录号分别是NP_727 563,NP_511 132,NP_727 564,NP_727 565),为85%。

According to the sequence of PCI246, two primers were designed. The 5＇end and 3＇ end of 70 ku heat shock protein cognate eDNA in crayfish（Procambarus clarkii） were amplified respectively, A 1 184 bp fragment was obtained with the completed sequence of 3＇end of the gene. The GenBank accession number is AY357302. The nucleotide identity between this protein and heat shock protein cognate of Bombyx mori was 83 %. And the amino acid identity between this protein and heat shock protein cognate 3 of Drosophila melanogaster was 85%.