两种产地的荆芥挥发油化学与药理的比较研究

Comparative Study on Chemidstry and Pharmacology of Schizonepetae Volatile Oil Produced in Different Places

目的:观察、比较不同产地荆芥挥发油的成分、药理作用的不同。方法:采用气质联用法进行挥发油化学成分分析;测定不同产地荆芥挥发油的半数致死量(LD50);二甲苯致小鼠耳廓肿胀试验观察不同产地荆芥挥发油的抗炎作用。结果:河北与湖南荆芥挥发油中薄荷酮和胡薄荷酮的比例分别为3.7∶1、1.1∶1;河北荆芥挥发油的LD50为(6.894±0.611)mL/kg,湖南样品为(0.842±0.092)mL/kg;河北样品0.4 mL/kg连续给药3天能显著对抗二甲苯所致小鼠耳廓肿胀,湖南样品同等剂量单次给药即表现出明显对抗作用。结论:不同产地荆芥挥发油中主要成分薄荷酮和胡薄荷酮比例的差异,可能造成其LD50、有效浓度及起效时间发生改变,但有待进一步探讨。

Objective： Observe and compare the chemical components and pharmacology action of Sto （Schizonepetae Volatile oil） which from different place of produce. Methods： The chemical components of Sto were determined by GC- MS. Through determining the LD50 to compare the toxicity of Sto which from different place of produce. Study the anti - inflammatory action of Sto by swelling of pinna induced by xylene in mice. Results： The ratio of menthol and pulegone in Sto that come from hebei and hunan was 3. 7 ： 1, 1. 1 ： 1 respectively. The LD50 of Sto that from Hebei was （6.894±0.611） ml/kg, meanwhile Hunan was （0.842±0.092） ml/kg. The sample from Hebei （0.4） ml/kg was intragastricly administrated for 3 days, significantly suppressed the swelling of pinna induced by xylene in mice. Meanwhile acute administration ofthe sample from Hunan showed the anti - inflammatory action in the same model in mice. Conclusion： Different place of produce of Sto maybe contribute to the difference in the ratio and level of main components, toxicity and pharmacodynamic action. Furthermore advanced investigation is needed.