RP-HPLC法测定维生素E霜的含量

Determination of Vitamin E in Vitamin E Cream by RP-HPLC

目的:测定维生素E霜中维生素E的含量。方法:采用反相高效液相色谱法,色谱柱:D iamonsilTM(钻石)C18柱,流动相:甲醇-氯仿(85∶15),流速:1.0 m l.m in-1,检测波长284 nm,柱温40℃。结果:维生素E的平均回收率为99.79%,方法精密度(RSD)为0.20%(n=6)。结论:该法可用于维生素E霜中维生素E的含量测定。

ObjectiveTo determine the content of Vitamin E in Vitamin E Cream. Methods RP - HPLC method was used. The chromatographic column was Diamonsil^TM C18, the mobile phase was methanol： chloroform （85： 15 ） , the flow rate was 1.0ml· min^-1 ,the detection wavelength was 284 nm, and the column temperature was 40 ℃. Results The average recoveries for Vitamin E was 99.79%, precision of the method was 0.20% （ RSD, n = 6）. Conclusion The method could be used to determine the content of Vitamin E in Vitamin E Cream.