气相色谱-质谱联用法测定水果中4,6-二硝基邻甲酚

Determination of dinitro-ortho-cresol in fruits by gas chromatography mass spectrometry

利用气质联用（GC-MS）技术建立了水果中4，6-二硝基邻甲酚（DNOC）的测定方法。以二氯甲烷提取水果样品中DNOC残留，并使用N-（特丁基二甲基硅）-N-甲基三氟乙酰胺对提取DNOC残留进行衍生化，利用GC-MS选择离子模式（SIM）测定残留DNOC衍生化产物，在苹果和梨中DNOC的添加回收率在70％～110％之间，相对标准偏差RSD〈9％（n=10），方法的检出限为10μg/kg。

The method for the determination of 4,6-dinitro-cresol （DNOC） in fruits has been established with GC-MS. Fristly, the residue of DNOC in fruits was extracted with dichloromethane and concentrated to dry under nitrogen, then DNOC was derivated by adding acetonitrile and derivatization reagent N-（t-butyldimethylsilyl）-N methyl-trifluoroacetamide （MTBSTFA）. Finally, derivatized DNOC in samples was analyzed by GC-MS with selected-ion monitoring （SIM）. The recoveries are from 70% to 110% and quantitative limit is l0 ktg/kg. The RSD is below 9% （n = 10）.