流动注射胶束化学发光法测定曲克芦丁

Determination of troxerutin by micellar chemiluminescence with flow-injection analysis

基于HCl介质中曲克芦丁与KIO4-MnSO4反应产生强的化学发光，且吐温-80的存在能使这一反应的化学发光强度大大增强。结合流动注射技术，建立了流动注射化学发光法测定曲克芦丁的新方法。在优化的实验条件下，曲克芦丁的质量浓度在2．0×10^-7～8．0×10^-5g／mE范围内与化学发光强度呈良好的线性关系．检出限为7×10^-8g／mL。对1．0×10^-6g／mL曲克芦丁进行11次平行测定，方法的相对标准偏差为1．8％。用于曲克芦丁片剂和针剂中曲克芦丁的测定。

A new flow-injection chemiluminescence method for the detennination of troxemtin with the system of KIO4- MnSO4-Troxemtin was established. Strong chemiluminescence signal was observed when the mixed solution of tween-80 and the sample was injected into KIO4 solution in a flow cell. Under the optimum conditions, the linear range for troxerutin was from 2.0×10^-7 to 8.0×10^-5g/mL and the detection limit was 7×10^-8 g/mL. The relative standard deviation was 1.8% （n = 11, ρ= 1.0×10^-6 g/mL）. This method has been successfully used for the determination of troxerutin in tablets and injections.