摘要
基于HCl介质中曲克芦丁与KIO4-MnSO4反应产生强的化学发光,且吐温-80的存在能使这一反应的化学发光强度大大增强。结合流动注射技术,建立了流动注射化学发光法测定曲克芦丁的新方法。在优化的实验条件下,曲克芦丁的质量浓度在2.0×10^-7~8.0×10^-5g/mE范围内与化学发光强度呈良好的线性关系.检出限为7×10^-8g/mL。对1.0×10^-6g/mL曲克芦丁进行11次平行测定,方法的相对标准偏差为1.8%。用于曲克芦丁片剂和针剂中曲克芦丁的测定。
A new flow-injection chemiluminescence method for the detennination of troxemtin with the system of KIO4- MnSO4-Troxemtin was established. Strong chemiluminescence signal was observed when the mixed solution of tween-80 and the sample was injected into KIO4 solution in a flow cell. Under the optimum conditions, the linear range for troxerutin was from 2.0×10^-7 to 8.0×10^-5g/mL and the detection limit was 7×10^-8 g/mL. The relative standard deviation was 1.8% (n = 11, ρ= 1.0×10^-6 g/mL). This method has been successfully used for the determination of troxerutin in tablets and injections.
出处
《分析试验室》
CAS
CSCD
北大核心
2006年第7期109-111,共3页
Chinese Journal of Analysis Laboratory
基金
延安大学科研课题项目(YDK2003-30)
关键词
化学发光
曲克芦丁
流动注射分析
Flow-injection analysis
Chemiluminesence
Troxemtin
Tween-80