p27^(kip1)基因在人胆管癌细胞化疗增敏效应中的作用

Sensitivities of Cholangiocarcinoma Cells Transfected by Exogenous p27^(kip1) Gene to Chemotherapy

目的探讨外源性p27kip1在人胆管癌细胞系QBC939中高表达对胆管癌细胞化疗敏感性的影响。方法通过携带人p27kip1基因的重组腺病毒载体Ad-p27mt转染人胆管癌细胞系QBC939。经逆转录-聚合酶链反应(RT-PCR)、Western blot检测p27kip1在胆管癌细胞中的表达;经MTT比色法及流式细胞术检测5-氟尿嘧啶(5-FU)、丝裂霉素(MMC)、环磷酰胺(CTX)对转染p27kip1前后的QBC939细胞生长抑制及凋亡的影响。结果Ad-p27mt转染后5-FU、MMC和CTX对QBC939细胞生长抑制率,分别由转染前的41.89%、45.59%和38.91%显著增加为56.15%、55.65%和51.69%;凋亡率也由13.76%、11.76%和10.46%,明显升高为41.39%、35.94%、34.46%,差异均有显著性意义(均P<0.05)。结论p27kip1在QBC939细胞中高表达,能显著增强胆管癌细胞对化疗药物的敏感性,为基因联合化疗药物治疗胆管癌提供了参考依据。

Objective To investigate the effects of exogenous p27^kip1 gene on chemosensitivity of human eholangiocarcinoma cell line. Methods The recombinant vector was constructed and the mutated p27^kip1 gene was transfected into human cholangiocarcinoma cell line （QBC939）. RT-PCR and Western blot were used to determine the expression of target genes. The effects of 5-fluorouracil （5-FU）, mitomycin （MMC） and cyclophosphamide （CTX） on the transfected cells were detected by assaying the rate of apoptosis and growth inhibition by methabenzthiazuron （MTT） assay and flow cytometry （FCM）. Results The exogenous p27^kip1 gene was expressed effectively in the cells, and the expression enhanced the apoptosis and growth inhibition of QBC939 induced by 5-FU, MMC and CTX. The ratio of growth inhibition was increased significantly from 41.89 % （5-FU）, 45.59 % （MMC） and 38.91% （CTX） to 56.15 % （5-FU）, 55.65 % （MMC） and 51.69 % （CTX） and apoptosis index from 13.76 % （5-FU）, 11.76 % （MMC）, 10.46 % （CTX） to 41.39 % （5-FU）, 35.94 % （MMC）, 34.46 % （CTX） respectively. Conclusion The exogenous p27^kip1 gene transfer can remarkably increase the drug sensibility of the cholangiocarcinoma cells. The combination of p27^kip1 gene therapy with chemotherapy may be more effective in cancer treatment.