电焊作业对工人血清一氧化氮合酶活力的影响

Effects of Welding on Activity of Serum Nitric Oxide Synthase among Welders

目的探讨电焊作业对工人血清中一氧化氮合酶(NOS)活力的影响,进一步了解电焊作业对工人健康损害的机制,为保护电焊作业工人身体健康提供理论依据。方法以某金属结构厂54名电焊作业工人为接触组,不接触毒物的59名非电焊工人为对照组,采用NOS催化L-Arg法测定血清中NOS活力;血锰含量测定采用石墨炉原子吸收法,血中铜、铁、锌、镁、钙含量测定采用火焰原子吸收法。结果接触组工人血清中总NOS活力为(27.88±7.49)U/ml,低于对照组(30.48±4.69)U/ml,差异有显著性(P<0.05),且接触组工人血清中诱导型NOS(iNOS)活力为(16.18±6.70)U/ml,亦低于对照组(19.71±4.26)U/ml,差异有显著性(P<0.05);血锰接触组为(42.73±21.63)μg/L,明显高于对照组(29.97±17.62)μg/L,差异有显著性(P<0.01);血锌接触组和对照组分别为(9.02±3.47)mg/L和(12.72±3.89)mg/L,两组间差异有显著性(P<0.05);接触组和对照组血镁分别为(47.99±9.86)mg/L和(35.82±15.08)mg/L,两组间差异有显著性(P<0.05);两组间血铜、血铁和血钙含量比较,差异均无显著性(P>0.05)。结论电焊作业可能导致工人血锰含量增加,而引起血清NOS活力降低;同时还可使血锌含量下降和血镁含量升高。

Objective To explore the effect of welding on activity of serum nitric oxide synthase （NOS） in welders and its hazardous mechanism. Methods The activity of serum NOS of 54 welders and 59 controls was determined by NOS catalyzed L-Arg method. The content of blood manganese was determined by graphite atomic absorption spectrophotometry. The contents of blood copper, iron, zinc, magnesium and calcium were determined by flame atomic absorption spectrophotometry. Results The activities of NOS and iNOS in welders were significantly lower than those of the controls （P〈0.05）. The contents of blood manganese and magnesium in welders were significantly higher than those of the controls（P〈0.01 or P〈0.05）. The content of blood zinc in welders was significantly lower than that of the controls （P〈0.05）. There was no significant difference in contents of blood copper, iron and calcium. Conclusions The content of blood manganese in welders could be increased, and the bioactivity of serum NOS and the content of blood zinc may be lowered, but the content of blood magnesium may be enhanced.