虎杖愈伤组织的诱导及其白藜芦醇形成初探

Callus Inducement and Resveratrol Formation of Polygonum cuspidatum

将虎杖P olygonum cusp id a tum不同外植体经不同消毒时间处理后,接种在添加不同激素种类和水平的相同基本培养基上或相同激素种类和水平基本培养基上进行诱导实验,同时对根和根茎芽、叶、韧皮诱导的愈伤组织进行白藜芦醇含量的测定.结果表明:基本培养基以M S较好,外植体叶对激素种类较为敏感,其中适当浓度的NAA诱导愈伤组织比2,4-D的效果好,KT比BA好,添加KT的培养基上诱导愈伤组织比较紧密,有利于分化,在M S+NAA 2 m g/L+KT 0.1 m g/L培养基上诱导愈伤组织较好,根茎芽的诱导率最高,为73%;愈伤组织的生长趋势从接种的第3天开始生长,到21 d时生长达到最高峰,干质量为0.461 2 g,以后生长速度减慢;对不同材料诱导的愈伤组织进行白藜芦醇含量的测定,其中根茎部芽的诱导的愈伤组织中白藜芦醇含量最高,其次是叶和根,最低的为韧皮.

The inducing experiments were carried out. Different explants of Polygonum cuspidatum after treatment of different antisepsis time were inoculated on the same elementary culture medium to which are added different kinds and levels of photohormones or on the different elementary culture medium to which are added the same kinds and levels of photohormones. The results show： MS is the best elementary culture medium; leaf explant was more sensitive to photohormone kinds ; effects on calli inducing NAA were better than 2,4-D; and KT were better than BA. Calli induced on the culture medium with KT were of high compactness that was quite beneficial to differentiation. Divided culture medium containing MS＋NAA 1.0 mg/L＋KT 1.5 mg/L had good effects of redivided onplant stem. Culture medium containing MS＋NAA 2 mg/L＋KT0.1 mg/L had good inducing effect on calli and its inducing rate on rhizome with bud was the highest, reaching up to 73%. The growing trend of callus began to grow three days after inoculation, and its growth came to the tiptop in the twenty-first day, but its growing speed became slow after its suttle reached 0. 461 2 g. A determination of the content of Resveratrol in the callus induced by different materials indicates the fact that the buds of rhizoma had the maximum content, the following being leaves and roots, and the bast having the minimum.