拟南芥悬浮细胞及其愈伤组织对潮霉素的反应

Impact of Hygromycin on Arabidopsis Suspension Cells and Calli

研究了潮霉素对拟南芥悬浮细胞及其愈伤组织的作用效果和特性.结果表明:潮霉素对拟南芥悬浮细胞生长、愈伤组织诱导及其生长均有极明显的抑制作用,但作用效果和特性均有所不同;拟南芥悬浮细胞继代培养阶段、愈伤组织诱导阶段和愈伤组织增殖阶段的临界致死质量浓度分别为25、2.5、1.5 m g/L,液体与固体培养条件下差异极大;潮霉素需要一定处理时间才能充分表现其抑制细胞生长或杀死细胞的效果,一般液体培养条件下3 d以后、固体培养条件下5 d以后效果趋于稳定,但不同的处理质量浓度有较大差异.还就液体与固体培养条件下作用效果差异的原因进行了讨论,并提出了对潮霉素抗性标记筛选的建议.

The impact of Hygromycin B on Arabidopsis suspension cells and calli was studied. The results show that the growth of Arabidopsis thaliana cells in vitro was significantly inhibited with Hygromycin B, and different effects and characteristics of inhibition were presented among different culture stages, especially between liquid and solid culture. On suspension subculture, calli inducement and calli subculture, the lethal critical concentrations of Hygromycin 13 for Arabidopsis thaliana cells were 25, 2. 5, 1. 5 mg/L in turn. A long or short time of treatment with Hygromycin B was necessary to impact on Arabidopsis thaliana cells fully. The inhibition efficiency became stable in 3 days in liquid and in 5 days on solid after treatment with Hygromycin B, but a considerable change presented among different concentrations. Furthermore, the probable causes of the different inhibition efficiency between liquid and solid culture conditions were discussed, and some suggestions were made for the selection of hygromycin-resistance marker on transformation of Arabidopsis suspension cells.