亚甲基四氢叶酸还原酶基因C677T多态性与儿童急性淋巴细胞白血病发病的关系

The relationship between the methylenetetrahydrofolate reductase C677T gene polymorphism and acute lymphocytic leukemia in children

目的:研究亚甲基四氢叶酸还原酶(Methylenetetrahytrofolate reductase,MTHFR)基因多态性与儿童急性淋巴细胞白血病(ALL)发病的关系。方法:对51例ALL儿童(ALL组)及53名健康儿童(对照组),采用PCR-RFLP技术检测两组MTHFR677C→T突变,统计两组的突变频率。结果:ALL组MTHFR677C→T多态性比率低于对照组(P<0．05)。结论:MTHFR基因677位碱基变异可能是中国儿童ALL的一个保护性因素。

Objective：To investigate the relationship between the gene polymorphism of methylenetetrahytrofolate reductase gene and the onset of childhood acute lymphocytic leukemia（ALL）. Method：The assay of polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was used to detect MTHFR gene C677T mutation in 53 healthy controls and 51 childhood patients with ALL. Result： The frequencies of MTHFR gene 677C→T polymorphism in patients were significantly lower than those in healthy controls （ P〈0.05）. Conclusion： The MTHFR gene C677T mutation may be a protective factor in the onset of childhood ALL.