骨形成蛋白在颌骨骨折愈合过程中的表达及mRNA水平分析

ExpresionandmRNATranscriptionofBoneMorphogeneticProtein(BMP)inFractureHealingofMandible

目的：观察骨形成蛋白（ＢＭＰ）在骨折愈合过程中的基因表达及发挥作用方式．方法：在建立兔颌骨骨折愈合模型的基础上，用免疫组织化学及原位杂交的方法在骨折愈合的不同时间分别检测ＢＭＰ表达产物和ＢＭＰ２ｍＲＮＡ转录水平．结果：在骨折愈合早期由受创伤的骨基质释放的ＢＭＰ在骨膜及骨断端骨髓中的骨诱导中发挥着重要的作用；此外，受到诱导刺激的细胞在分化为具有成骨及成软骨能力的细胞的同时，也具有合成及分泌ＢＭＰ的能力，这种能力保证了正常骨组织的形成．结论：本实验结果进一步证实了ＢＭＰ骨诱导贯穿于骨折愈合整个过程。

Aim:Theaimofpresentstudywastoobservethepaternsofexpressionofbonemorphogeneticprotein(BMP)geneandfunctionofBMPintheprocessesofbonefracturehealing.Methods:TheexpressingproductionandthetranscriptionallevelofBMP2mRNAinaseriesofintervalsoftheestablishedfracturehealingmodeloftherabbitmandibleweredetectedbyimmunohistochemistryandinsituhybridization.Results:TheresultsdemonstratedthattheBMPreleasedfromtheinjuredbonematrixinitiatedtheboneinductionsofperiosteumandbonemarrowintheearlystageoffracturehealing;inaddition,thecelswhichwereeitherinducedbyBMPorstimulatedbyotherfactors,wereabletosynthesizeandsecreteBMPwhiletheywereundergoingthediferentiationintotheosteogeneticand/orchondro-geneticcels.Conclusion:Theresultsofourstudyfurtherindicatethattheprocessesofbonefracturehealingareaccom-paniedbythepresenceofBMP-boneinductionandthatBMPplaysasignificantroleinosteogenesisduringfractureheal-ing.