同种异体骨移植免疫与骨诱导的相关研究

ACorrelativeStudyontheImmuneRejectionandOsteoinductiveCapacityofBoneAlograft

目的：探讨同种异体骨移植免疫排异反应与移植物骨诱导活性内在相互作用．方法：新鲜骨、自消化抗原去除骨、骨基质明胶、脱钙骨基质分别植入小鼠肌肉，行免疫学、组织学和碱性磷酸酶检测．结果：４种同种异体骨移植物均诱发免疫排异，以新鲜骨最强烈，骨基质明胶最弱．自消化抗原去除骨、骨基质明胶和脱钙骨基质抑制体外淋巴细胞增殖，以骨基质明胶的抑制作用最显著．自消化抗原去除骨、骨基质明胶和脱钙骨基质均在体内诱导异位成骨，骨基质明胶的骨诱导活性最佳．

Aim:Tostudythecorrelationshipbetweentheimmunerejectionandtheosteoinductivepotentialofbonealograft.Methods:Alogeneicandsyngeneicfreshbone(FB),autolyzedantigen-extractedbone(AAA),bonematrixgelatin(BMG),demineralizedbonematrix(DBM)wereimplantedintothemuscleofthemice,andimmunologicaltests,histologicalobservationandalkalinephosphatase(ALP)assaywereperformed.Results:Alkindsofalogeneicimplantsactivatedimmunerejection.Amongthem,FBinducedthemostvigorousimmunerejectionandBMGcausedtheweakestresponse.AlogeneicAAA,BMGandDBMinhibitedproliferationofthelymphocytesinvitroandBMGhadthemostpowerfulinhibitingefect.BothalogeneicandsyngeneicBMG,DBMandAAAinducedheterotopicosteogenesisinvivoandBMGhadthebestosteoinductivecapacity.Conclusion:Thereisnegativecorrelationbetweenimmunerejectiontobonealograftandostoinductivecapacityofthegraft.