摘要
目的:比较烧伤、冲击伤和烧冲复合伤时肺微血管内皮细胞的损伤情况.方法:用ELISA和免疫组化结合半定量的方法检测了正常及伤后血浆血管性假血友病因子(vWF)含量和肺微血管内皮细胞vWF含量积分变化.结果:各致伤组伤后血浆vWF含量均较正常对照组明显增多(P<0.01),复合伤组最明显;而肺微血管内皮细胞vWF含量积分则较对照明显减少(P<0.01),冲击伤和复合伤组血浆vWF增多与肺微血管vWF含量减少呈显著负相关关系,烧伤组两指标变化相关不显著.
Aim:Tocomparethedamageofpulmonarymicrovascularendothelialcelsafterburns,blastinjuryandburn-blastinjury.Methods:TheplasmavonWilebrandfactor(vWF)contentsandvWFscoresofpulmonarymicrovas-cularendothelialcels(EC)wereassayedusingELISAandimmunohistochemicalanalysisinrats.Results:TheplasmavWFcontentineachinjuredgroupwasincreasedmoresignificantlythanthatincontrolone(P<0.01),andthevWFscoreofpulmonarymicrovascularECineachinjuredgroupwasdecreasedmoresignificantlythanthatincontrolone(P<0.01).TherewasaclosedandnegativecorrelationbetweentheincreaseofplasmavWFcontentsandthedecreaseofvWFscoresofpulmonarymicrovascularECintheblastinjuredgroupandburn-blastcombinedinjuredgroup(P<0.01).Conclusion:ThedamagetopulmonarymicrovascularECinburn-blastinjuryismoreseverethanthatinburnsorblastinjury.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
1996年第6期366-368,共3页
Chinese Journal of Trauma
关键词
血管性
假血友病因子
内皮细胞
冲击伤
烧伤
vonWilebrandfactorEndothelialcelBlastinjuryBurnsBurn-blastinjury