烧伤、冲击伤和烧冲复合伤对肺微血管内皮细胞损伤的比较

AlterationsofPulmonaryMicrovascularEndothelialCelsafterBurns,BlastInjuryandBurn┐BlastCombinedInjury:AComparativeStudy

目的：比较烧伤、冲击伤和烧冲复合伤时肺微血管内皮细胞的损伤情况．方法：用ＥＬＩＳＡ和免疫组化结合半定量的方法检测了正常及伤后血浆血管性假血友病因子（ｖＷＦ）含量和肺微血管内皮细胞ｖＷＦ含量积分变化．结果：各致伤组伤后血浆ｖＷＦ含量均较正常对照组明显增多（Ｐ＜０．０１），复合伤组最明显；而肺微血管内皮细胞ｖＷＦ含量积分则较对照明显减少（Ｐ＜０．０１），冲击伤和复合伤组血浆ｖＷＦ增多与肺微血管ｖＷＦ含量减少呈显著负相关关系，烧伤组两指标变化相关不显著．

Aim:Tocomparethedamageofpulmonarymicrovascularendothelialcelsafterburns,blastinjuryandburn-blastinjury.Methods:TheplasmavonWilebrandfactor(vWF)contentsandvWFscoresofpulmonarymicrovas-cularendothelialcels(EC)wereassayedusingELISAandimmunohistochemicalanalysisinrats.Results:TheplasmavWFcontentineachinjuredgroupwasincreasedmoresignificantlythanthatincontrolone(P<0.01),andthevWFscoreofpulmonarymicrovascularECineachinjuredgroupwasdecreasedmoresignificantlythanthatincontrolone(P<0.01).TherewasaclosedandnegativecorrelationbetweentheincreaseofplasmavWFcontentsandthedecreaseofvWFscoresofpulmonarymicrovascularECintheblastinjuredgroupandburn-blastcombinedinjuredgroup(P<0.01).Conclusion:ThedamagetopulmonarymicrovascularECinburn-blastinjuryismoreseverethanthatinburnsorblastinjury.