摘要
目的:建立高效液相色谱法测定清肺抑火片中栀子苷的含量。方法:采用Diamonsil C18柱(250mm×4.6mm,5μm);以水-乙腈(85:15)为流动相;检测波长为238nm;流速为0.8ml/min。结果:栀子苷在0.1504-0.7520μg范围内呈良好线性关系(r=0.9995);平均回收率为99.6%,RSD为1.1%。结论:本法快速、准确、重现性好,可用于清肺抑火片中栀子苷的含量测定。
CT Object: An HPLC method was developed for the determination of geniposide in Qingfei yihuo tablets. Methods: The chromatographic separation was carried out on a Diamonsil Cis column(250 mm ×4.6 mm, 5 μm)with the mobile phase consisting of water and acetonitrile (85: 15,v/v). Detection wavelength was set at 238 nm; The flow rate was 0.8 ml/min. Results: The linear range were 0.150 4 -0.752 0 μg ( r = 0.999 5 ) ; The average recovery was 99.6% with RSD of 1.1%. Conclusion: The method was rapid, accurate and reproducible. It was suitable for the content detemination of geniposide in Qingfei yihuo tablets.
出处
《天津药学》
2006年第3期16-17,共2页
Tianjin Pharmacy