气相色谱法测定尿素维E乳膏中维生素E的含量

Determination of vitamin E in urea and vitamin E cream by GC

目的建立一种用气相色谱法测定尿素维E乳膏中维生素E含量的方法。方法采用硅酮OV—17填充柱（涂布浓度为2％）；柱温：275℃；进样口温度：295℃；FID检测器，检测器温度320℃。结果维生素E在2．00—6．06μg范围内呈良好线性关系（r=0．9992），平均加样回收率为97．1％，RSD为1．4％（n=9）。结论该法简便快速，结果准确，可以有效地控制该药品的质量。

Aim To estabilsh a method for determining vitamin E in urea and vitamin E cream by GC. Method 2% OV-17 packed column. The column temperature was at 275℃. Injector temperature was at 295℃ ,FID detector with temperature at 320℃. Results Vitamin E showed a good linear relationship at a range of 2,130 -6,061μg（r =0.9992）. The average recovery and the relative standard deviation was 97.1% and 1. 4% respectively. Conclusion The method was convenient and rapid. It was accurate and very suitable for the quality control of the drug.