消除Ia阳性细胞及短程应用CyA诱导大鼠甲状旁腺同种异体移植物长期存活

Tolerance to Rat Parathyroid Allograft Induced by Depletion of Ia￣+ Donor Cells Plus Short Course Cyclosporine

本实验采用单抗加补体方法消除大鼠甲状旁腺（ＰＴＧ）移植物中的Ｉａ阳性细胞，处理后移植物分泌功能保存良好。将其移植到同种受体肾包膜下，移植物平均存活时间达６０ｄ，与未处理移植组（１４ｄ）相比，Ｐ＜０．０１。若受鼠接受移植前应用环孢素（ＣｙＡ）３ｄ（２．５ｍｇ·ｋｇ^（－１）／ｄ），则可使６０％经处理的ＰＴＧ同种异体移植物存活期大于１５０ｄ；与未处理短程应用ＣｙＡ组（７０ｄ）相比，Ｐ＜０．０１。说明消除Ｉａ抗原阳性细胞与术前短程给予受体ＣｙＡ联合应用，可使大鼠ＰＴＧ同种异体移植物获得长期存活。

In this study,Ia￣+ donor cells of rat parathyroid allografts were depleted by anti-Ia monoclonal antibody plus complement.The secreting function of treated glands as well preserved.Then we transplanted the treated glands into subcapsular of the recipient kindey,and the median survival time(MST) of the allografts were 60 days, compared with 14 daysin the fresh PTG group(P<0.01 ).If the recipients received 25 mg/kg cyclosponine forthree days before transplantation,the MST of about 60%PTG allografts was more than 150days,with P<0.01 in comparison with the control group(short course CyA alone,MST:70days ). These results indicate that long-term survival of rat parathyroid allografts can beachived by depletion of la￣+ donor cells and short course pretreatment of the recipient withCyA。