耐甲氧西林凝固酶阴性葡萄球菌抗菌药物及消毒剂耐药基因研究

Drug-resistant and Antiseptic-resistant Genes of Productive Enzyme in Meticillin-resistant Coagulase Negative Staphylococcus

目的了解耐甲氧西林凝固酶阴性葡萄球菌(MRCNS)抗菌药物及消毒剂耐药基因流行病学状况。方法对40株MRCNS菌进行mecA、aac(6′)/aph(2″)、aph(3′)-Ⅲ、ermA/B/C、tetM、qacA/B检测。结果40株MRCNS菌mecA、aac(6′)/aph(2″)、aph(3′)-Ⅲ、ermA/B/C、tetM、qacA/B基因阳性株分别为39、32、15、30、2、6株,阳性率分别为97.5%、80.0%、37.5%、75.0%、5.0%、15.0%;其中同时检出mecA基因、aac(6′)/aph(2″)/和(或)aph(3′)-Ⅲ基因、ermA/B/C基因26株(65.0%)。结论65.0%MRCNS菌已同时携带mecA基因、aac(6′)/aph(2″)/或aph(3′)-Ⅲ基因、ermA/B/C基因。

OBJECTIVE To study the epidemiology of drug-resistant and antiseptic-resistant genes of productive enzyme in meticillin-resistant coagulase negative Staphylococcus （MRCNS）. METHODS mecA Gene of the drugresistant β-lactam, aac （6′）/aph （2″） and aph （3′）-Ⅲ genes of aminoglycoside-modifying enzyme （AME） and ermA/B/C genes of erythromycin methyltransferase were detected by polymerase chain reaction （PCR） in 40 MRCNS strains. RESULTS From them there were 39 strains with mecA gene, 32 strains with aac（6′）/aph（2＂） gene, 15 strains with aph（3′） -HI gene, 30 strains with ermA/B/C gene, 2 strains with tetM and 6 strains with qacA/B gene. There were 26 strains （65.0%） simultaneously with mecA, aac（6′）/aph（2＂）and （or） aph（3′）-HI and ermA/B/C genes in MRCNS. CONCLUSIONS The drug-resistant rate is higher in MRCNS. There are more than half tested strains simultaneously with 3 to 4 drug-resistant genes.