铜中毒对雏鸡外周血T-淋巴细胞的影响(英文)

Effect of Copper Toxicity on Peripheral Blood T-lymphocyte in the Chicken

180只1日龄艾维茵肉鸡健雏随机分为3组，分别饲喂对照（每千克日粮含铜11．97mg）和铜中毒（每千克日粮舍铜650mg，铜中毒Ⅰ组；每千克日粮含铜850mg，铜中毒Ⅱ组）日粮6周，以流式细胞术和酸性-α-醋酸萘酯酶（ANAE）染色的方法观测外周血T-淋巴细胞的动态变化。2个铜中毒组1～6周龄外周血T-淋巴细胞的ANAE阳性率均显著低于对照组（P〈0．05或P〈0．01），同时2个铜中毒组间比较也呈显著差异（P〈0．05或P〈0．01）。与对照组比较，2个铜中毒组2～6周龄CD4^＋T淋巴细胞数量均显著降低（P〈0．05或P〈0．01），CD8^＋T淋巴细胞数量变化不明显（P〉0．05），CD4^＋／CD8^＋比值降低（P〈0．05）。结果表明，铜中毒可抑制T-淋巴细胞的生成，降低其在外周血中的数量。

The experiment was conducted to examine the effect of copper toxicity on the peripheral blood T-lymphocyte using the flow cytometry（FCM） and ANAE. 180 one-day-old Avian broilers were divided randomly into three groups,and fed diets as follows：（1）Controls（Cu 11.97 mg/kg diet） and （2）copper toxic（Cu 650 mg/kg diet,copper toxic group Ⅰ ;Cu 850 mg/ kg diet ,copper toxic group Ⅱ ） for six weeks. The ANAE positive ratios of the peripheral blood T-lymphocytes were much lower in the two copper toxic groups than in control group from 1 to 6 weeks of age（P〈0.05 or P〈0. 01 ）. Also ,there was s;gnificant difference between copper toxic groups Ⅰ and Ⅱ at 1,3,5 and 6 weeks of age（P〈0.05 or P〈0. 01）. CD4^＋ T cell numbers reduced from 2 to 6 weeks of age in both copper toxic group Ⅰ and copper toxic group Ⅱ as compared with those of control group （P〈0. 05 or P〈0. 01）. At the same time,there was significant difference between copper toxic groups Ⅰ and Ⅱ at 6 weeks of age（P〈0.05）. But the numbers of CD8^＋ T cell were not varied from 2 to 6 weeks of age in copper toxic groups Ⅰ and Ⅱ in comparison with those of control group（P〉0. 05）. The CD4^＋/CD8^＋ ratio was lower from 2 to 6 weeks of age in copper toxic groups Ⅰ and Ⅱ than in control group. The results showed that copper toxicity could suppress the development of T-lymphocytes and reduce the peripheral blood T-lymphocyte populations. Potential mechanisms underlying these observations are also discussed.