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New Approach to Detecting Phenoxyl Free Radicals Generated in Enzyme Reaction by Stopped-flow Spectrophotometry

New Approach to Detecting Phenoxyl Free Radicals Generated in Enzyme Reaction by Stopped-flow Spectrophotometry
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摘要 A highly sensitive stopped-flow spectrophotometry kinetic method was proposed for quantification phenoxyl radicals based on their accelerating effect on the oxidation of nicotinamide adenine dinucleotide (NADH). Phenoxyl radicals generated from as low as 1×10^-8 mol/L 2,4-DCP can be readily detected with the proposed method and the detecting limit was 2.5×10^-9 mol/L. A highly sensitive stopped-flow spectrophotometry kinetic method was proposed for quantification phenoxyl radicals based on their accelerating effect on the oxidation of nicotinamide adenine dinucleotide (NADH). Phenoxyl radicals generated from as low as 1×10^-8 mol/L 2,4-DCP can be readily detected with the proposed method and the detecting limit was 2.5×10^-9 mol/L.
出处 《Chinese Chemical Letters》 SCIE CAS CSCD 2006年第7期949-952,共4页 中国化学快报(英文版)
基金 The authors gratefully acknowledge financial support from the National Natural Science Foundation of China (No. 20275027 and No. 20377032).
关键词 Phenoxyl radicals moleculary probe stopped-flow spectrophotometry. Phenoxyl radicals, moleculary probe, stopped-flow spectrophotometry.
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