RP-HPLC测定天然虫草与人工虫草中核苷类成分的含量

Determination of Nucleosides in Natural Cordyceps Sinensis and Cultured Cordyceps by RP-HPLC

目的 分析不同来源的天然虫草与人工虫草中主要核苷类成分的差异。方法 应用RP-HPLC检测18个样品中尿苷、腺嘌呤、腺苷、虫草素的含量。Symmetry Shield Rp18柱（3．9mm×150mm，5um），以乙腈-水为流动相，梯度洗脱。流速0．2～1．2mL·min^-1，检测波长260nm，柱温30℃。结果 本法高效灵敏，重现性好。所测人工虫草核苷类成分的含量较天然虫草高，不同产地天然虫草4种主要的核苷类成分的差异较不同来源人工虫草的小。结论 本法能有效鉴别虫草的核苷类成分，可用于天然虫草和人工虫草中核苷类成分的分析，评价不同来源虫草的质量。

OBJECTIVE To develop a HPLC method for the determination of the nucleosids from Cordyceps sinensis and cultured Cordyceps, and to study the difference in them.METHODS Eighteen samples of Cordycep from different resources were collected. Uridine, adenine, adenosine, and cordycepin were determined by RP-HPLC method. The separation was performed on a Symmetry Shield Rpl8 （3.9 mm × 150 mm,5 μm） with UV detection at 260 nm, the mobile phase used were acetonitrile-water with gradient elution, the flow rate was from 0.2 ～.2 mL·min^-1 and the columm temperature was controlled at 30℃. RESULTS This method was accurate and reproducible as well as specific. The contents of nuleosides from cultured Cordyceps were higher than that of those from wildlife. The differences of every constituent content among the four active nucleosides in Cordyceps sinensis were lower than those in cultured Cordyceps. CONCLUSION This method effectively distinguished nucleosides in various Cordyceps. Consequently it can be used to analyze nucleosides between Cordyceps sinensis and cultured Cordyceps as well as evaluate their quality.