摘要
目的:建立一种高效液相色谱法测定茚地那韦胶囊含量及有关物质的方法。方法:色谱柱为 C_8柱;流动相为0.02mol·L^(-1)枸橼酸钠缓冲液(用稀盐酸调 pH 至4.0)-乙腈(45:55);检测波长为260nm;流速为1.0mL·min^(-1)。结果:茚地那韦在0.153~0.612mg·mL^(-1)的范围内,峰面积与浓度呈线性关系,r=0.9999(n=7),平均回收率为100.01%,RSD 为0.68%。结论:用 HPLC 法测定茚地那韦胶囊含量及有关物质方法简便,结果准确。
Objective:A HPLC methods was established for the determination of Indinavir capsules and its related substances. Method :The column : shim - pack Alhima Cs column ( 250 mm × 4. 6 mm 5 μm). The mobile phase : 0. 02 mol · L 6- 1 citrate buffer - acetonitrile (45 : 55 ). The detective wavelength : 260 nm. The flow rate : 1.0 mL · min^-1. Result:standard curve was linear in the range of 0. 153 -0. 612 mg · mL^-1 and thecorrelation coefficient was 0. 9999. The average recovery was100. 01% (RSD = 0. 68%, n = 9). Conclusion:The method is easy to operate and the results of the determination is accurate.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第6期860-861,共2页
Chinese Journal of Pharmaceutical Analysis