摘要
BoLA-DRB3基因是主要组织相容性复合物(Major Histocompatibility Complex,MHC)基因家族中的Ⅱ类基因,它是该基因家族中最主要的功能基因,所编码的MHC抗原与免疫应答、抗病性密切相关。其第2外显子是编码抗原的主要功能区。本试验以地方良种鲁西牛、秦川牛、晋南牛和南阳牛为研究对象,采用PCR-RFLP方法对DRB3基因307bp的扩增产物进行多态性分析,结果表明DRB3基因第2外显子的第154位C→A,从而产生新的等位基因。经χ2适合性检验,鲁西牛在MHC-DRB3基因第2外显子的MspⅠ酶切位点未达到Hardy-Wein-berg平衡状态(P<0.05)。
BoLA-DRB3 is a member of the MHC （Major Histocompatibility Complex）, which plays a central role in immune system and disease resistance of bovine. The exon2 of BoLA-DRB3 gene is a mainly functional region, which codes the antigen and participates in the body＇s immune response. In this study, the 307bp fragment including the exon2 in Luxi , Qinchuan, Nanyang and Jinnan cattle was amplified by polymerase chain reaction, which was digested with restriction endonuclease Msp I , The results showed new allele was produced by the mutation of C to A at position 154 in exon2. Statistical results of X2 test indicated that genetic polymorphism sites of the exon2 of BoLA-DRB3 locus in Luxi cattle did not fit Hardy-Weinberg equilibrium （P〈0. 05）.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2006年第7期722-726,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
"863"国家高技术研究发展计划项目(2002AA242011)
国家农业科技跨越计划项目(2004跨20)
