乳酸菌对牙鲆稚鱼养殖水体和肠道菌群的影响

Effects of lactic acid bacteria on the microflora in water and larvae guts of Japanese flounder (Paralichthys olivaceus )

在牙鲆稚鱼中投喂添加由单一鼠李糖乳杆菌P15制备的微生态制剂和黄霉素,在60d的投喂期内,采用平板计数法检测好氧性异养菌的总数、总弧菌数和乳酸菌的数量。结果表明,在投喂菌液和冻干菌粉后,养殖水体和牙鲆肠道的乳酸菌数均呈上升趋势,在30d后乳酸菌数量达到稳定并在肠道内定植。同时,由于乳酸菌的抑制作用,弧菌的数量下降,以肠道中的弧菌最明显。乳酸菌对养殖水体和牙鲆肠道的好氧性异养菌没任何影响。乳酸菌对养殖水体和肠道菌群的影响与抗生素具有相近的效果,表明乳酸菌作为饲料添加剂可以取代抗生素应用在牙鲆的养殖中。

Japanese flounder （Paralichthys olivaceus） larvae were reared on feed supplement with Lactobacillus rharnnosus P15 alone and flavomycin. After 60 days of feeding, the total numbers of aerobes, vibrios, and lactic acid bacteria （LAB） were measured by plate colony count. The result showed that the numbers of LAB rose in both culture water and intestine of the flounder administrated with suspended and lyophilized bacteria. After 30 days, LAB colo- nized in the flounder gut with stable number. Meanwhile, the number of vibrios reduced, especially in the flounder gut, owing to the inhibitory effect of LAB. LAB had no effects on aerobes in culture water and Japanese flounder gut. The influences of LAB on the microflora in culture water and Japanese flounder gut were similar to those of flavomycin. It was suggested that LAB additive could be used in Japanese flounder culture as substitute for antibiotics.