普仑司特对实验性变应性鼻炎鼻黏膜组织重塑的影响

Effect of Pranlukast on tissue remodeling in experimental guinea pig allergic rhinitis model

目的观察半胱氨酸白三烯受体拮抗剂——普仑司特(pranlukast)对变应性鼻炎动物模型鼻黏膜组织重塑的影响。方法Hartley豚鼠14只,随机分为3组,即阴性对照组、卵清蛋白(ovalbumin,OVA)组和OVA+普仑司特组。OVA组:致敏的豚鼠经鼻长期OVA激发共12周;OVA +普仑司特组:致敏的豚鼠经鼻抗原激发1周后,抗原激发的同时给予普仑司特腹腔注射11周;阴性对照组仅给予生理盐水腹腔注射。标本石蜡包埋,做苏木素-伊红、阿辛蓝-过碘酸-希夫(alcian blue- periodic acid-Schiff,AB-PAS)和Masson三色胶原(Masson Trichrome,MT)染色,计数鼻中隔黏膜中的嗜酸粒细胞、上皮杯状细胞数量,半定量测量上皮细胞损伤及鼻中隔黏膜和鼻甲基底膜区及上皮下胶原含量,测定数值以x±s表示。结果与阴性对照相比,OVA组发生鼻中隔黏膜嗜酸粒细胞浸润[400倍镜下细胞数,(106．90±13．66)个／镜下]、上皮细胞损伤(完整上皮占47．25％±7．67％)、杯状细胞化生[每毫米基底膜对应上皮细胞中杯状细胞数,(22．05±5．81)个／mm]和鼻中隔黏膜及鼻甲内细胞外基质沉积明显增多;OVA+普仑司特组未见明显鼻中隔黏膜嗜酸粒细胞浸润[(8．95±2．32)个／镜下]、上皮细胞损伤(完整上皮占83．15％±8．05％),杯状细胞化生[(5．73±1．07)个／mm]以及鼻中隔黏膜及鼻甲内细胞外基质沉积。OVA+普仑司特组与阴性对照组差异无统计学意义。结论普仑司特能够阻止长期抗原激发导致的鼻黏膜组织重塑的发生,早期应用普仑司特对变应性鼻炎的治疗具有一定意义。

Objective To explore the impact of Pranlukast in nasal mucosal remodeling in experimental allergic rhinitis. Methods Foureteen male guinea pigs were randomly divided into 3 groups： control group, ovalhumin （OVA） group and OVA ＋ Pranlukast group. In the OVA group and OVA ＋ Pranlukast group, OVA sensitized Hartley guinea pigs were exposured intranasally to OVA for a total of 12 weeks, the OVA ＋ Pranlukast group received additional Pranlukast treatment from the second week to the 12th week. Paraffine embeded sections were stained with hematoxylin and eosin （ HE）, alcian blue-periodic acid-Schiff （AB-PAS）, and Masson＇s Trichrome（MT）. Infiltrating eosinophils, the number of goblet cells in the surface epithelium and gland cells in subepithelial nasal septal mucosa were counted. The damage of epithelium in nasal septum and extracellular matrix of nasal septal mucosa and conchae were determined. Results Compared with the control, the prolonged OVA exposure protocol caused significant pathological changes in the nasal mucosa, which included eosinophils infiltration into epithelium and submucosa（ 106. 90 ±13.66）, significant goblet hyperplalsia （22.05 ±5.81/mm）, epithelial damage （intact epithelium： 47. 25%±7. 67% ） and deposition of extracellular matrix. These changes were significantly inhibited by Pranlukast, in which group, there were few eosinophils（ 8.95 ± 2. 32） , few goblet cells（5.73±1.07/mm）, and relative intact epithelium（ intact epithelium： 83. 15% ± 8.05% ） , and no significant ECM deposition. Conclusions Early Pranlukast intervansion could inhibit nasal mucosal remodeling in allergic rhinitis.