Tumor differentiation phenotype in gastric differentiated-type tumors and its relation to tumor invasion and genetic alterations

AIM： To clarify the relations between tumor differentiation phenotype and tumor invasion or genetic alterations in gastric differentiated-type tumors. METHODS： We examined the tumor differentiation phenotype, the presence of mutations in APC and p53, and the microsatellite instability （MSI） status in 48 gastric adenomas and 171 differentiated-type carcinomas, The tumor differentiation phenotype was determined by examining the expression of human gastric mucin （HGM）, NUC6, MUC2 and CD10, The tumors were then classified into gastric- （G-）, gastric and intestinal mixed （GI-）, or intestinal- （I-） phenotypes, according to the immunopositivity of the above markers, The presence of mutations in APC and p53 and the MSI status were also investigated in all the tumors, RESULTS： Gastric adenomas were significantly associated with CDIO expression, I-phenotype tumors and the presence of APC mutations, compared with carcinomas （66.7% vs 25.1%, P 〈 0.0001; 56.3% vs 14.6%, P 〈 0.0001; 39.6% vs 14.0%, P 〈 0.0001, respectively） and inversely associated with expressions of HGM and MUC6 and the presence of p53 mutations （10.4% vs 62.6%, P 〈 0.0001; 39.6% vs 64.3%, P = 0.003; 2.0% vs 26.3%, P = 0.001, respectively）. The frequency of APC mutations was significantly higher in HGM-negative tumors, MUC6-negative tumors, CD10-positive tumors and I-phenotype tumors than in HGM-positive tumors, MUC6- positive tumors, CD10-negative tumors and G-phenotype tumors （32.7% vs 7.1%, P 〈 0.0001; 27.8% vs 14.0%, P = 0.0182; 37.3% vs 10.4%, P 〈 0.0001; and 38.5% vs 9.5%, P = 0.0017, respectively）. The frequency of MSI was significantly higher in MUC6-positive tumors, CD10- negative tumors and G-phenotype tumors than in MUC6- negative tumors, CD10-positive tumors and I-phenotype tumors （24.8% vs 6.7%, P = 0.0009; 22.2% vs 8.0%, P = 0.0143; and 28.6% vs 9.6%, P = 0.0353, respectively）. CONCLUSION： The tumor differentiation phenotype is closely related to tumor invasion and genetic alterations in gastric differentiated-type tumors.

瞄准：在胃的区分类型的肿瘤澄清在肿瘤区别显型和肿瘤侵略或基因改变之间的关系。方法：我们检验了肿瘤区别在 48 个胃的腺瘤和 171 区分类型的癌的显型，在 APC 和 p53 的变化的存在，和微卫星不稳定性(MSI ) 地位。肿瘤区别显型被检验人的表示决定胃粘蛋白(HGM ) ， MUC6， MUC2 和 CD10。肿瘤然后被分类进胃 --(G-) ，胃、肠混合 --( 官方补给 --) ，或肠 --( 我 --) 显型，根据上述标记的免疫确实。在 APC 和 p53 和 MSI 地位的变化的存在也在所有肿瘤被调查。结果：胃的腺瘤显著地与 CD10 表示，我显型肿瘤和 APC 变化的存在被联系，与癌相比(66.7% 对 25.1% ， P < 0.0001；56.3% 对 14.6% ， P < 0.0001；39.6% 对 14.0% ， P < 0.0001，分别地) 并且相反地与 HGM 和 MUC6 和 p53 变化的存在的表情联系了(10.4% 对 62.6% ， P < 0.0001；39.6% 对 64.3% ， P = 0.003；2.0% 对 26.3% ， P = 0.001，分别地) 。APC 变化的频率在比在 HGM 积极的肿瘤， MUC6 积极的肿瘤， CD10 否定的肿瘤和 G 显型肿瘤的 HGM 否定的肿瘤， MUC6 否定的肿瘤， CD10 积极的肿瘤和我显型肿瘤是显著地更高的(32.7% 对 7.1% ， P < 0.0001；27.8% 对 14.0% ， P = 0.0182；37.3% 对 10.4% ， P < 0.0001；并且 38.5% 对 9.5% ， P = 0.0017，分别地) 。MSI 的频率在比在 MUC6 否定的肿瘤， CD10 积极的肿瘤和我显型肿瘤的 MUC6 积极的肿瘤， CD10 否定的肿瘤和 G 显型肿瘤是显著地更高的(24.8% 对 6.7% ， P = 0.0009；22.2% 对 8.0% ， P = 0.0143；并且 28.6% 对 9.6% ， P = 0.0353，分别地) 。结论：肿瘤区别显型是仔细与在胃的区分类型的肿瘤的肿瘤侵略和基因改变有关。