单味黄芪红花丹参注射液对神经干细胞分化影响的初步研究

Effect of simple astragali,flos carthami,salvia miltiorrhiza injection on differentiation of neural stem cells

目的寻找对神经干细胞(neural stem cells,NSCs)的增殖、分化和存活有促进作用的单味中药成分,进而确定对NSCs的增殖和分化有影响的中药有效成分。方法本研究筛选了黄芪、红花、丹参3种中药注射液分别刺激培养的NSCs,观察NSCs的分化状态。结果发现黄芪、红花有明显的促进NSCs分化的作用,其中黄芪效果最佳,而丹参却有促进NSCs凋亡的作用,这种作用随着其浓度的升高而加强。结论黄芪、红花对NSCs的分化起积极作用,丹参对NSCs的分化有毒性作用,也可能促进NSCs的凋亡,为进一步从细胞水平和分子水平研究中药的作用机制和NSCs的分化机制提供了依据。

Objective To find a simple traditional Chinese medicine that certainly activates the proliferation, differentiation and survival of neural stem cells （NSCs）. Methods The parenteral solution of astragali, flos carthami or salvia mihiorrhiza was used to stimulate NSCs in vitro and the differentiation of NSCs was observed. Results Astragali and flos carthami could induce the differentiation of NSCs and the effect of astragali was better. Salvia mihiorrhiza could induce the apoptosis of NSCs in a concentration-dependent manner. Conclusion Astragli and flos carthami were found to accelerate transparently NSCs to differentiate, but salvia miltiorrhiza had toxic effect on differntiation of NSCs.