心导管留置术对大鼠脏器功能影响的实验研究

Effect of heart catheterization on organ function in rat

目的观察心导管留置术对大鼠血培养和脏器形态、功能的影响,为临床前药理研究制备稳定可靠的动物模型。方法采用改良的心导管留置术建立大鼠模型,每24小时定时采血作血培养并检测血白细胞(WBC)和丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)、尿素氮(BUN)、肌酐(Cr)、肌酸磷酸激酶(CK)等生化指标和血浆内毒素(LPS)水平,术后7 d取心、肝、肾作病理形态学观察。结果术后大鼠血培养均未检出细菌,血中WBC、ALT、AST、BUN、Cr及LPS基本在正常范围内,CK值变化较大但术后第7天恢复正常。心、肝、肾无病理学改变。结论此心导管留置术对大鼠感染和脏器功能影响小,动物模型稳定可靠。

Objective To observe the impact of heart catheterization on blood and organ function, and create an stable animal model. Methods Ten male Wistar rats were divided into control group undergoing sham operation and experimental group undergoing improved heart catheterization （n =5 in each group）. Blood samples were obtained every day from 10 rats before and after operation, and white blood cell （WBC） , alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, blood urea nitrogen （BUN）, creatinine （Cr）, creatine phosphokinase （CK） and lipopolysaccharide were detected. The pathomorphology of heart, liver and kidney in catheterized rats was observed on postoperative day 7. Results For the catheterized rats, blood cultures were negative of bacteria and the markers above were within normal range except for CK that recovered to normal value in 7 d, while the control rats had no obvious damage. Conclusion Heart catheterization causes no infection and organ function changes in rats. The animal model of heart catheterization for clinical pharmacological research is reliable.