摘要
目的观察转化生长因子β1(transforminggrowthfactorβ1,TGFβ1)对体外培养的角膜成纤维细胞结缔组织生长因子(connectivetissuegrowthfactor,CTGF)的诱导表达作用。方法体外培养的角膜成纤维细胞分实验组和对照组,对照组弃原培养液,加无血清培养液继续培养,实验组用不同浓度的TGFβ1(0.1μg·L-1、1μg·L-1、10μg·L-1)处理24h,用RTPCR方法检测角膜成纤维细胞CTGFmRNA的表达情况。结果1μg·L-1和10μg·L-1TGFβ1处理组CTGF/GAPDH像素比值分别为0.35±0.05和1.25±0.10,均较对照组高(0.13±0.02),差异具有统计学意义,同时CTGF/GAPDH比值随TGFβ1浓度增加而增加。结论TGFβ1可以诱导角膜成纤维细胞CTGF的表达,在角膜成纤维细胞中CTGF也可能是TGFβ1对细胞起作用的下游信号分子。
Objective To study the induction of TGF-β1 on CTGF expression in cultured corneal fibroblasts.Methods Cultured rabbit corneal fibroblasts were divided into experimental group and control group. In control group, the primary DMEM was deserted and DMEM containing no fetal bovine serum was added. In the experimental group the corneal fibroblasts were treated with 0.1 μg· L^-1,1 μg· L^-1 and 10μg· L^-1 TGF-β1 respectively for 24 hours, then RTPCR was used to detect CTGF mRNA expressions. Results The values of CTGF/GAPDH ratio of the cells in the experimental group treated with 1μg· L^-1 TGF-β1 and 10 μg· L^-1 TGF-1β were 0.35 ± 0.05 and 1.25 ± 0.10 respectively, high- er than that of control group （0.13 ± 0.02）, which showed a statistical significance. While the concentration of TGF-β1 was increased, the value of CTGF/ GAPDH ratio was enhanced. Conclusion TGF-β1 can induce CTGF expression in cultured corneal fibroblasts which shows that CTGF also could be a downstream signaling molecule when TGF-β1 acts on corneal fibroblasts. [Rec Adv Ophthalmol 2006;26（7） ：508-510]
出处
《眼科新进展》
CAS
2006年第7期508-510,共3页
Recent Advances in Ophthalmology
