碱性成纤维细胞生长因子基因对猪缺血心肌侧支血管生成的作用

Therapeutic angiogenesis with the use of basic fibroblast growth factor gene in the ischemic myocardium of swine

目的探讨经导管冠状动脉内注射碱性成纤维细胞生长因子(bFGF)基因(pcDNA3-bFGF)对猪缺血心肌侧支血管生成的作用。方法实验猪分为3组:手术对照组(n=6)、bFGF基因左冠状动脉注射组(n=6)和bFGF基因右冠状动脉注射组(n=6)。开胸结扎冠状动脉左回旋支(LCX),建立急性心肌梗死动物模型。术后2周,分别行选择性冠状动脉造影,并经导管冠状动脉内注射pcD-NA3-bFGF 2 000μg。给药后2周,再次行选择性冠状动脉造影观察冠状动脉侧支血管形成情况,并通过病理切片光镜观察、免疫组化染色进行血管计数,观察猪缺血心肌侧支血管生成情况。结果(1)病理切片及免疫组化染色结果显示左、右冠状动脉注射基因组血管计数较对照组明显增加;(2)术后4周选择性冠状动脉造影显示左、右冠状动脉注射基因组侧支血管明显多于对照组,左冠状动脉注射基因组侧支血管多于右冠状动脉基因注射组。结论经导管冠状动脉内注射bFGF基因能促进猪缺血心肌侧支血管生成。

Objective To study the effects of basic fibroblast growth factor gene in enhancing collateral vessel formation of coronary artery in the ischemic myocardium of swine. Methods All swines were distributed into the operation control group （ n = 6） and treatment group. The treatment group was further divided into 2 groups according to the route of injection either through the right coronary artery or the left coronary （ n = 6 respectively）. The animal models of AMI were prepared by ligating the left circumflex （LCX） coronary artery. Two weeks after the operation, 2 000 μg of pcDNA3-bFGF eukaryotic expression plasmid was directly injected into the coronary artery by catheter. Two weeks after the gene injection, evaluation of collateral circulation formation was made by means of coronary angiography and immuohistological staining ect. Results （1） Through immuotistological staining, the vessels count in both treatment groups was more than that in the control group; （2） Selective coronary angiography at 4 weeks after the operation showed that the number of newly formed collateral vessels in the bFGF gene treatment group was more than that in the control group. On the other hand, more collateral vessels were found in the group through left coronary injection compared with the group through right coronary injection. Conclusion Intra-coronary artery injection of bFGF gene can improve collateral vascular formation in the ischemic myocardium of swine.